CRISPR/Cas system and factors affecting its precision and efficiency
The diverse applications of genetically modified cells and organisms require more precise
and efficient genome-editing tool such as clustered regularly interspaced short palindromic …
and efficient genome-editing tool such as clustered regularly interspaced short palindromic …
Dynamics and mechanisms of CRISPR-Cas9 through the lens of computational methods
The clustered regularly interspaced short palindromic repeat (CRISPR) genome-editing
revolution established the beginning of a new era in life sciences. Here, we review the role …
revolution established the beginning of a new era in life sciences. Here, we review the role …
Improved gRNA secondary structures allow editing of target sites resistant to CRISPR-Cas9 cleavage
S Riesenberg, N Helmbrecht, P Kanis, T Maricic… - Nature …, 2022 - nature.com
The first step in CRISPR-Cas9-mediated genome editing is the cleavage of target DNA
sequences that are complementary to so-called spacer sequences in CRISPR guide RNAs …
sequences that are complementary to so-called spacer sequences in CRISPR guide RNAs …
Massively parallel kinetic profiling of natural and engineered CRISPR nucleases
Engineered SpCas9s and AsCas12a cleave fewer off-target genomic sites than natural
SpCas9. However, understanding their fidelity, mechanisms and cleavage outcomes …
SpCas9. However, understanding their fidelity, mechanisms and cleavage outcomes …
Bridge helix arginines play a critical role in Cas9 sensitivity to mismatches
M Bratovič, I Fonfara, K Chylinski, EJC Gálvez… - Nature Chemical …, 2020 - nature.com
The RNA-programmable DNA-endonuclease Cas9 is widely used for genome engineering,
where a high degree of specificity is required. To investigate which features of Cas9 …
where a high degree of specificity is required. To investigate which features of Cas9 …
Comprehensive deletion landscape of CRISPR-Cas9 identifies minimal RNA-guided DNA-binding modules
A Shams, SA Higgins, C Fellmann, TG Laughlin… - Nature …, 2021 - nature.com
Proteins evolve through the modular rearrangement of elements known as domains. Extant,
multidomain proteins are hypothesized to be the result of domain accretion, but there has …
multidomain proteins are hypothesized to be the result of domain accretion, but there has …
Leveraging QM/MM and Molecular Dynamics Simulations to Decipher the Reaction Mechanism of the Cas9 HNH Domain to Investigate Off-Target Effects
Y Maghsoud, VM Jayasinghe-Arachchige… - Journal of Chemical …, 2023 - ACS Publications
The clustered regularly interspaced short palindromic repeats (CRISPR) technology is an
RNA-guided targeted genome-editing tool using Cas family proteins. Two magnesium …
RNA-guided targeted genome-editing tool using Cas family proteins. Two magnesium …
Coordinated actions of Cas9 HNH and RuvC nuclease domains are regulated by the bridge helix and the target DNA sequence
CRISPR-Cas systems are RNA-guided nucleases that provide adaptive immune protection
in bacteria and archaea against intruding genomic materials. Cas9, a type-II CRISPR …
in bacteria and archaea against intruding genomic materials. Cas9, a type-II CRISPR …
Molecular mechanism of D1135E-induced discriminated CRISPR-Cas9 PAM recognition
M Kang, Z Zuo, Z Yin, J Gu - Journal of Chemical Information and …, 2022 - ACS Publications
The off-target effects of Streptococcus pyogenes Cas9 (SpCas9) pose a significant
challenge to harness it as a therapeutical approach. Two major factors can result in SpCas9 …
challenge to harness it as a therapeutical approach. Two major factors can result in SpCas9 …
Rational engineering of CRISPR-Cas9 nuclease to attenuate position-dependent off-target effects
The RNA-guided Cas9 nuclease from Streptococcus pyogenes has become an important
gene-editing tool. However, its intrinsic off-target activity is a major challenge for biomedical …
gene-editing tool. However, its intrinsic off-target activity is a major challenge for biomedical …