Zinc proteins are an integral component of the proteome of all domains of life. Zn (ii), one of
the most widespread transition elements, serves multiple functions in proteins, such as a …

Abstract Metallo-β-lactamases (MBLs) are zinc-dependent hydrolases that inactivate
virtually all β-lactam antibiotics. The expression of MBLs by Gram-negative bacteria severely …

Understanding the evolution of metallo-β-lactamases (MBLs) is fundamental to deciphering
the mechanistic basis of resistance to carbapenems in pathogenic and opportunistic …

Co (II) is sometimes utilized as a spectroscopically active substitute for Zn (II) in enzymes.
Metal binding sites in enzymes that contain catalytically active Zn (II) generally yield high …

An electrothermal, near-torch vaporization (NTV) sample introduction for micro-or nano-
volume samples is described. Samples were pipetted onto coiled-filament assemblies that …

The ability, either innate or acquired, to produce β-lactamases, enzymes capable of
hydrolyzing the endocyclic peptide bond in β-lactam antibiotics, would appear to be a …

ABSTRACT IMP-type metallo-β-lactamases are di-Zn (II) enzymes that can inactivate a wide
range of bicyclic β-lactam agents used in clinical practice. IMP-27 shares 82% amino acid …

Metals are the most commonly encountered protein cofactors, and they play important
structural and functional roles in biology. In many cases, metal binding provides a major …

A Re coil-filament in-torch vaporization (ITV) sample introduction system was interfaced to a
sector field inductively coupled plasma-mass spectrometry (SFICP-MS) system. In this first …

Microbial proteases are significant enzymes acquiring 60% of the enzyme market. The
heterologous expression provides higher enzymatic yield, yet such proteases possess less …