Three-step docking by WIPI2, ATG16L1, and ATG3 delivers LC3 to the phagophore
The covalent attachment of ubiquitin-like LC3 proteins (microtubule-associated proteins
1A/1B light chain 3) prepares the autophagic membrane for cargo recruitment. We resolve …
1A/1B light chain 3) prepares the autophagic membrane for cargo recruitment. We resolve …
Membrane association of the ATG8 conjugation machinery emerges as a key regulatory feature for autophagosome biogenesis
Autophagy is a highly conserved intracellular pathway that is essential for survival in all
eukaryotes. In healthy cells, autophagy is used to remove damaged intracellular …
eukaryotes. In healthy cells, autophagy is used to remove damaged intracellular …
Molecular free energies, rates, and mechanisms from data-efficient path sampling simulations
Molecular dynamics is a powerful tool for studying the thermodynamics and kinetics of
complex molecular events. However, these simulations can rarely sample the required time …
complex molecular events. However, these simulations can rarely sample the required time …
Atg8ylation as a host-protective mechanism against Mycobacterium tuberculosis
V Deretic - Frontiers in tuberculosis, 2023 - frontiersin.org
Nearly two decades have passed since the first report on autophagy acting as a cell-
autonomous defense against Mycobacterium tuberculosis. This helped usher a new area of …
autonomous defense against Mycobacterium tuberculosis. This helped usher a new area of …
[HTML][HTML] The N-terminal region of the ATG8 autophagy protein LC3C is essential for its membrane fusion properties
U Ballesteros, MN Iriondo, YR Varela, FM Goñi… - International Journal of …, 2024 - Elsevier
Autophagy is a catabolic process in which a double-membrane organelle, the
autophagosome (AP), engulfs cellular components that will be degraded in the lysosomes …
autophagosome (AP), engulfs cellular components that will be degraded in the lysosomes …
When an underdog becomes a major player: the role of protein structural disorder in the Atg8 conjugation system
H Popelka, DJ Klionsky - Autophagy, 2024 - Taylor & Francis
The noncanonical ubiquitin-like conjugation cascade involving the E1 (Atg7), E2 (Atg3,
Atg10), and E3 (Atg12–Atg5-Atg16 complex) enzymes is essential for incorporation of Atg8 …
Atg10), and E3 (Atg12–Atg5-Atg16 complex) enzymes is essential for incorporation of Atg8 …
Membrane atg8ylation in canonical and noncanonical autophagy
Membrane atg8ylation is a homeostatic process responding to membrane remodeling and
stress signals. Membranes are atg8ylated by mammalian ATG8 ubiquitin-like proteins …
stress signals. Membranes are atg8ylated by mammalian ATG8 ubiquitin-like proteins …
ATG3 proteins possess a unique amphipathic α-helix essential for the Atg8/LC3 lipidation reaction
In our recent paper, we uncovered that ATG3 exhibits a large degree of structural dynamics
on autophagic membranes to efficiently carry out LC3 lipidation. ATG3 proteins possess an …
on autophagic membranes to efficiently carry out LC3 lipidation. ATG3 proteins possess an …
Three-step docking by WIPI2, ATG16L1 and ATG3 delivers LC3 to the phagophore
The covalent attachment of ubiquitin-like LC3 proteins prepares the autophagic membrane
for cargo recruitment. We resolve key steps in LC3 lipidation by combining molecular …
for cargo recruitment. We resolve key steps in LC3 lipidation by combining molecular …
Structural biology of the Atg8 and Atg12 conjugation systems
NN Noda - Autophagy Reports, 2023 - Taylor & Francis
Atg8 and Atg12 are ubiquitin-like proteins, conjugated to phosphatidylethanolamine (PE)
and Atg5, respectively, through enzymatic reactions similar to ubiquitylation. The resultant …
and Atg5, respectively, through enzymatic reactions similar to ubiquitylation. The resultant …