Past, present, and future of affinity-based cell separation technologies

K Bacon, A Lavoie, BM Rao, M Daniele, S Menegatti - Acta biomaterialia, 2020 - Elsevier
Progress in cell purification technology is critical to increase the availability of viable cells for
therapeutic, diagnostic, and research applications. A variety of techniques are now available …

Economic evaluation of the primary recovery of tetracycline with traditional and novel aqueous two-phase systems

MA Torres-Acosta, JFB Pereira, MG Freire… - Separation and …, 2018 - Elsevier
Antibiotics are a key pharmaceutical to inhibit growth or kill microorganisms. They represent
a profitable market and, in particular, tetracycline has been listed as an essential medicine …

Generation of polyethylene glycol-dextran aqueous two-phase system droplets using different culture media under in vitro conditions

K Chairez-Cantu, M González-González… - Food and Bioproducts …, 2023 - Elsevier
Aqueous two-phase systems (ATPS) involve the combination of two immiscible polymers
that at certain concentrations form a biphasic separation system. Due to the physicochemical …

Characterization of polymer‐polymer aqueous two‐phase system droplets for 3D culture future applications

K Chairez‐Cantu… - Journal of Chemical …, 2023 - Wiley Online Library
BACKGROUND Cell‐based high‐throughput platforms using aqueous two‐phase systems
(ATPS) have led to the construction of 3D cultures using ATPS in which stem cells are …

Influence of tie line length and volume ratio on the partition behavior of peripheral blood and conjugated CD34 antibody in polymer-polymer aqueous two-phase …

M Nisslein, M González-González… - Separation and …, 2021 - Elsevier
Aqueous two-phase systems (ATPS) offer great opportunities regarding the purification of
stem cells for future medical applications. However, most publications exploring the use of …

Aqueous two-phase systems for the recovery and purification of phage therapy products: Recovery of salmonella bacteriophage ϕSan23 as a case study

V Clavijo, MA Torres-Acosta, MJ Vives-Flórez… - Separation and …, 2019 - Elsevier
Contamination with Salmonella can cause significant economic and public health problems.
Its prevention is accomplished by the use of antibiotics, but appearance of resistant strains …

Monolithic chromatography: insights and practical perspectives

M González‐González… - Journal of Chemical …, 2017 - Wiley Online Library
The use of monoliths, one‐piece porous separation materials, as column packaging media
is known as monolithic chromatography and this technology has been widely exploited to …

Application of affinity aqueous two‐phase systems for the fractionation of CD133+ stem cells from human umbilical cord blood

M González‐González… - Journal of Molecular …, 2015 - Wiley Online Library
In a further attempt to establish a novel stem cell primary recovery strategy, the use of
aqueous two‐phase systems (ATPS) complemented with the use of antibodies (known as …

Aqueous two‐phase systems as a novel alternative in the primary recovery of human mesenchymal cells obtained from adipose tissue

D Freire‐Flores, N Llanovarced‐Kawles… - Journal of Chemical …, 2024 - Wiley Online Library
In recent years, therapies based on regenerative medicine have gained wide interest owing
to their potential in organ transplantation and in the treatment of multiple diseases. These …

Elimination of contaminants from cell preparations using aqueous two-phase partitioning

M González-González, RC Willson… - Separation and …, 2016 - Elsevier
Contamination by cell debris and non-viable cells is common in cell-based processes. Cell
fragments and dead cells can be produced during culture and in purification steps, and often …