Conspectus Cytochrome P450 monooxygenase is a versatile oxidizing enzyme with great
potential in synthetic chemistry and biology. However, the dependence of its catalytic …

Cytochrome P450s are heme-thiolate enzymes that have been broadly applied in
pharmaceutical and biosynthesis because of their efficient oxidation at inert carbons …

Given prominent physicochemical similarities between H2O2 and water, we report a new
strategy for promoting the peroxygenase activity of P450 enzymes by engineering their …

In recent years, the engineering of flexible loops to improve enzyme properties has gained
attention in biocatalysis. Herein, we report a loop engineering strategy to improve the …

Esterification is one of the most important reactions in organic synthesis and industrial
applications. The applications of lipases in esterification are increasingly widespread and …

Cytochrome P450 BM3 monooxygenase is the topic of extensive research as many
researchers have evolved this enzyme to generate a variety of products. However, the …

Cytochrome P450s are versatile catalysts for biosynthesis applications. In the P450 catalytic
cycle, two electrons are required to reduce the heme iron and activate the subsequent …

Abstract Cytochrome P450 monooxygenases (CYPs/P450s) are heme-thiolate proteins with
broad chemical functionality that have widespread application as powerful biocatalysts …

Abstract Cytochrome P450 (CYP450s) as a large superfamily of ubiquitous heme-containing
monooxygenases are widely distributed in kingdoms of life, catalyzing versatile reactions …

Understanding structure–function relationships in proteins is pivotal in their development as
industrial biocatalysts. In this regard, rational engineering of protein active site access …