The discovery, mechanisms, and evolutionary impact of anti-CRISPRs
AL Borges, AR Davidson… - Annual review of …, 2017 - annualreviews.org
Bacteria and archaea use CRISPR-Cas adaptive immune systems to defend themselves
from infection by bacteriophages (phages). These RNA-guided nucleases are powerful …
from infection by bacteriophages (phages). These RNA-guided nucleases are powerful …
The influence of eukaryotic chromatin state on CRISPR–Cas9 editing efficiencies
SAN Verkuijl, MG Rots - Current opinion in biotechnology, 2019 - Elsevier
Highlights•CRISPR/Cas9 is the method of choice for site-directed genetic or transcriptional
manipulation.•Genomic loci differ in chromatin configuration which affects Cas9 …
manipulation.•Genomic loci differ in chromatin configuration which affects Cas9 …
[HTML][HTML] Structural basis for Cas9 off-target activity
The target DNA specificity of the CRISPR-associated genome editor nuclease Cas9 is
determined by complementarity to a 20-nucleotide segment in its guide RNA. However …
determined by complementarity to a 20-nucleotide segment in its guide RNA. However …
Engineering modular viral scaffolds for targeted bacterial population editing
Bacteria are central to human health and disease, but existing tools to edit microbial
consortia are limited. For example, broad-spectrum antibiotics are unable to precisely …
consortia are limited. For example, broad-spectrum antibiotics are unable to precisely …
Phage-encoded anti-CRISPR defenses
SY Stanley, KL Maxwell - Annual review of genetics, 2018 - annualreviews.org
The battle for survival between bacteria and bacteriophages (phages) is an arms race where
bacteria develop defenses to protect themselves from phages and phages evolve …
bacteria develop defenses to protect themselves from phages and phages evolve …
Discovery of potent and versatile CRISPR–Cas9 inhibitors engineered for chemically controllable genome editing
Anti-CRISPR (Acr) proteins are encoded by many mobile genetic elements (MGEs) such as
phages and plasmids to combat CRISPR–Cas adaptive immune systems employed by …
phages and plasmids to combat CRISPR–Cas adaptive immune systems employed by …
Engineering of bacteriophage T4 genome using CRISPR-Cas9
Bacteriophages likely constitute the largest biomass on Earth. However, very few phage
genomes have been well-characterized, the tailed phage T4 genome being one of them …
genomes have been well-characterized, the tailed phage T4 genome being one of them …
Keeping crispr in check: diverse mechanisms of phage-encoded anti-crisprs
D Trasanidou, AS Geros, P Mohanraju… - FEMS microbiology …, 2019 - academic.oup.com
ABSTRACT CRISPR-Cas represents the only adaptive immune system of prokaryotes
known to date. These immune systems are widespread among bacteria and archaea, and …
known to date. These immune systems are widespread among bacteria and archaea, and …
[HTML][HTML] Functional genomics of the lactic acid bacterium Limosilactobacillus fermentum LAB-1: metabolic, probiotic and biotechnological perspectives
TJ Hossain - Heliyon, 2022 - cell.com
A genome-based systematic analysis was conducted to characterize the metabolic,
probiotic, fitness, and safety properties of Limosilactobacillus fermentum LAB-1, a lactic acid …
probiotic, fitness, and safety properties of Limosilactobacillus fermentum LAB-1, a lactic acid …
Optimization of T4 phage engineering via CRISPR/Cas9
A major limitation hindering the widespread use of synthetic phages in medical and
industrial settings is the lack of an efficient phage-engineering platform. Classical T4 phage …
industrial settings is the lack of an efficient phage-engineering platform. Classical T4 phage …