Recent advances in droplet microfluidics for single-cell analysis

Z Jiang, H Shi, X Tang, J Qin - TrAC Trends in Analytical Chemistry, 2023 - Elsevier
Single-cell manipulation and analysis is essential for studying many fundamental biological
processes and discovering cellular heterogeneity. Multi-parameter studies of intercellular …

Emerging droplet microfluidics

L Shang, Y Cheng, Y Zhao - Chemical reviews, 2017 - ACS Publications
Droplet microfluidics generates and manipulates discrete droplets through immiscible
multiphase flows inside microchannels. Due to its remarkable advantages, droplet …

Passive and active droplet generation with microfluidics: a review

P Zhu, L Wang - Lab on a Chip, 2017 - pubs.rsc.org
Precise and effective control of droplet generation is critical for applications of droplet
microfluidics ranging from materials synthesis to lab-on-a-chip systems. Methods for droplet …

Ultrahigh-Throughput Enzyme Engineering and Discovery in In Vitro Compartments

M Gantz, S Neun, EJ Medcalf, LD van Vliet… - Chemical …, 2023 - ACS Publications
Novel and improved biocatalysts are increasingly sourced from libraries via experimental
screening. The success of such campaigns is crucially dependent on the number of …

Single molecule protein detection with attomolar sensitivity using droplet digital enzyme-linked immunosorbent assay

L Cohen, N Cui, Y Cai, PM Garden, X Li, DA Weitz… - ACS …, 2020 - ACS Publications
Many proteins are present at low concentrations in biological samples, and therefore,
techniques for ultrasensitive protein detection are necessary. To overcome challenges with …

Future of digital assays to resolve clinical heterogeneity of single extracellular vesicles

RTT Morales, J Ko - ACS nano, 2022 - ACS Publications
Extracellular vesicles (EVs) are complex lipid membrane vehicles with variable expressions
of molecular cargo, composed of diverse subpopulations that participate in the intercellular …

Single-exosome-counting immunoassays for cancer diagnostics

C Liu, X Xu, B Li, B Situ, W Pan, Y Hu, T An, S Yao… - Nano …, 2018 - ACS Publications
Exosomes shed by tumor cells have been recognized as promising biomarkers for cancer
diagnostics due to their unique composition and functions. Quantification of low …

Cancer biomarker detection: recent achievements and challenges

L Wu, X Qu - Chemical Society Reviews, 2015 - pubs.rsc.org
The early detection of cancer can significantly reduce cancer mortality and saves lives. Thus,
a great deal of effort has been devoted to the exploration of new technologies to detect early …

The Poisson distribution and beyond: methods for microfluidic droplet production and single cell encapsulation

DJ Collins, A Neild, A DeMello, AQ Liu, Y Ai - Lab on a Chip, 2015 - pubs.rsc.org
There is a recognized and growing need for rapid and efficient cell assays, where the size of
microfluidic devices lend themselves to the manipulation of cellular populations down to the …

Controlled droplet microfluidic systems for multistep chemical and biological assays

TS Kaminski, P Garstecki - Chemical Society Reviews, 2017 - pubs.rsc.org
Droplet microfluidics is a relatively new and rapidly evolving field of science focused on
studying the hydrodynamics and properties of biphasic flows at the microscale, and on the …