Native mass spectrometry of membrane proteins
Biological membranes formed of amphipathic lipid bilayers provide chemical barriers to
separate cells from their external environments and define subcellular compartments …
separate cells from their external environments and define subcellular compartments …
[HTML][HTML] Peroxiredoxins wear many hats: Factors that fashion their peroxide sensing personalities
Peroxiredoxins (Prdxs) sense and assess peroxide levels, and signal through protein
interactions. Understanding the role of the multiple structural and post-translational …
interactions. Understanding the role of the multiple structural and post-translational …
Microfluidic devices for protein analysis using intact and top‐down mass spectrometry
Top‐down mass spectrometry (TD‐MS) provides unique information on compositions,
structures, and functions of proteins or protein complexes and has been recognized as a …
structures, and functions of proteins or protein complexes and has been recognized as a …
Rapid profiling of the glycosylation effects on the binding of SARS-CoV-2 spike protein to angiotensin-converting enzyme 2 using MALDI-MS with high mass detection
Y Zhou, C Tan, R Zenobi - Analytical Chemistry, 2024 - ACS Publications
The spike protein receptor-binding domain (RBD) of SARS-CoV-2 binds directly to
angiotensin-converting enzyme 2 (ACE2), mediating the host cell entry of SARS-CoV-2 …
angiotensin-converting enzyme 2 (ACE2), mediating the host cell entry of SARS-CoV-2 …
Combination of continuous wavelet transform and genetic algorithm-based Otsu for efficient mass spectrometry peak detection
J Zhou, J Li, W Gao, S Zhang, C Wang, J Lin… - Biochemical and …, 2022 - Elsevier
Mass spectrometry (MS) data is susceptible to random noises and alternating baseline,
posing great challenges to spectral peak detection, especially for weak peaks and …
posing great challenges to spectral peak detection, especially for weak peaks and …
Development of a Dual-Epitope Nanobody-Based Immunosensor with MXenes@ CNTs@ AuNPs for Ultrasensitive Detection of Rotavirus
W Hu, S Yang, X Wang, X Li, L Lei, H Lin… - Analytical …, 2024 - ACS Publications
Immunoassays have become essential tools for detecting infectious viruses. However,
traditional monoclonal antibody-dependent immunoassays are costly, fragile, and unstable …
traditional monoclonal antibody-dependent immunoassays are costly, fragile, and unstable …
What's the defect? Using mass defects to study oligomerization of membrane proteins and peptides in nanodiscs with native mass spectrometry
JA Townsend, MT Marty - Methods, 2023 - Elsevier
Many membrane proteins form functional complexes that are either homo-or hetero-
oligomeric. However, it is challenging to characterize membrane protein oligomerization in …
oligomeric. However, it is challenging to characterize membrane protein oligomerization in …
High-Mass Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry for Absolute Quantitation of Noncovalent Protein–Protein Binding Interactions
Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is a robust and
powerful tool for studying biomacromolecules and their interactions. However, quantitative …
powerful tool for studying biomacromolecules and their interactions. However, quantitative …
Insight to functional conformation and noncovalent interactions of protein-protein assembly using MALDI mass spectrometry
M Giampà, E Sgobba - Molecules, 2020 - mdpi.com
Noncovalent interactions are the keys to the structural organization of biomolecule eg,
proteins, glycans, lipids in the process of molecular recognition processes eg, enzyme …
proteins, glycans, lipids in the process of molecular recognition processes eg, enzyme …
Native Agarose Gels and Contact Blotting as Means to Optimize the Protocols for the Formation of Antigen–Ligand Complexes
C D'Ercole, A De Marco - Bioengineering, 2023 - mdpi.com
Background: Protein complexes provide valuable biological information, but can be difficult
to handle. Therefore, technical advancements designed to improve their manipulation are …
to handle. Therefore, technical advancements designed to improve their manipulation are …