Immunotherapies that block inhibitory checkpoint receptors on T cells have transformed the
clinical care of patients with cancer. However, whether the T cell response to checkpoint …

In this review, we discuss the development pipeline for transcriptional biomarkers in
molecular diagnostics and stress the importance of a reliable gene transcript quantification …

Xyloglucans are highly substituted and recalcitrant polysaccharides found in the primary cell
walls of vascular plants, acting as a barrier against pathogens. Here, we reveal that the …

The development of 'age clocks', machine learning models predicting age from biological
data, has been a major milestone in the search for reliable markers of biological age and …

SUMMARY Soybean (Glycine max [L.] Merr.) is a major crop in animal feed and human
nutrition, mainly for its rich protein and oil contents. The remarkable rise in soybean …

Gene expression signatures have been critical in defining the molecular phenotypes of cells,
tissues, and patient samples. Their most notable and widespread clinical application is …

Multiple statistical approaches have been proposed to validate reference genes in qPCR
assays. However, conflicting results from these statistical methods pose a major hurdle in …

Gene expression in apple roots in response to various stress conditions is a less-explored
research subject. Reliable reference genes for normalizing quantitative gene expression …

Quantitative PCR (qPCR) remains the most widely used technique for gene expression
evaluation. Obtaining reliable data using this method requires reference genes (RGs) with …

Potato (Solanum tuberosum L.) is the world's most crucial non-cereal food crop and ranks
third in food production after wheat and rice. Despite the availability of several potato …