Multiplexed, single-molecule, epigenetic analysis of plasma-isolated nucleosomes for cancer diagnostics

V Fedyuk, N Erez, N Furth, O Beresh… - Nature …, 2023 - nature.com
The analysis of cell-free DNA (cfDNA) in plasma provides information on pathological
processes in the body. Blood cfDNA is in the form of nucleosomes, which maintain their …

Single-cell analysis of the epitranscriptome: RNA modifications under the microscope

E Crespo-García, A Bueno-Costa, M Esteller - RNA biology, 2024 - Taylor & Francis
The identification of mechanisms capable of modifying genetic information by the addition of
covalent RNA modifications distinguishes a level of complexity in gene expression which …

Infection Meets Inflammation: N6-Methyladenosine, an Internal Messenger RNA Modification as a Tool for Pharmacological Regulation of Host–Pathogen Interactions

MN Leseva, B Buttari, L Saso, PA Dimitrova - Biomolecules, 2023 - mdpi.com
The significance of internal mRNA modifications for the modulation of transcript stability, for
regulation of nuclear export and translation efficiency, and their role in suppressing innate …

In situ visualization of m6A sites in cellular mRNAs

CJ Sheehan, BF Marayati, J Bhatia… - Nucleic Acids …, 2023 - academic.oup.com
Abstract N 6-methyladenosine (m6A) is an abundant RNA modification which plays critical
roles in RNA function and cellular physiology. However, our understanding of how m6A is …

A review of advances in analytical strategies for RNA methylation

H Sun, F Yin, Z Zou, Y Gu, C Guo - Analytica Chimica Acta, 2024 - Elsevier
Abstract N6-methyladenosine (m 6 A) is the most prevalent RNA methylation modification,
and its dysregulation causes aberrant transcription and translation, which plays critical roles …

Recent advance in optical single-molecule detection of methylation modification and methyl-modifying enzymes

Y Han, D Yang, S Jiang, S Zhao, F Ma… - TrAC Trends in Analytical …, 2024 - Elsevier
As the predominant epigenetic markers, methyl-modifying enzymes-catalyzed methylation
modification widely participates in the regulation of cellular functions, and the dysregulation …

Visualization and Quantification of Single‐Base m6A Methylation

Q Zhang, Y Dai, X Teng, J Li - … Chemie International Edition, 2024 - Wiley Online Library
N 6‐methyladenosine (m6A) has emerged as the most prevalent form of RNA modification
found across various RNA classes. The detection and quantification of m6A RNA …

One-Base-Gap Circular Probe-Mediated Dual Amplification for Isothermal Detection of N6-Methyladenosine Modifications

X Ma, Y Xia, S Wang, Z Yang, X Lei, Y Wu… - Analytical …, 2023 - ACS Publications
N6-Methyladenosine (m6A) stands out as the predominant internal modification in
mammalian RNA, exerting crucial regulatory functions in the metabolism of mRNA. Currently …

The role of RNA modifications in disease-associated macrophages

C Huart, MS Gupta, JA Van Ginderachter - Molecular Therapy-Nucleic Acids, 2024 - cell.com
In recent years, the field of epitranscriptomics has witnessed significant breakthroughs with
the identification of more than 150 different chemical modifications in different RNA species …

Statistical modeling of single-cell epitranscriptomics enabled trajectory and regulatory inference of RNA methylation

H Wang, Y Wang, J Zhou, B Song, G Tu, A Nguyen… - Cell genomics, 2024 - cell.com
As a fundamental mechanism for gene expression regulation, post-transcriptional RNA
methylation plays versatile roles in various biological processes and disease mechanisms …