Our understanding of the genetic mechanisms that underlie biological processes has relied
extensively on loss-of-function (LOF) analyses. LOF methods target DNA, RNA or protein to …

The use of zebrafish in functional genomics and disease modeling has become popular due
to the ease of targeted mutagenesis with genome editing nucleases, ie, zinc finger …

Generating transgenic and gene edited mammals involves in vitro manipulation of oocytes
or single cell embryos. Due to the comparative inaccessibility of avian oocytes and single …

Abstract Main conclusion The CRISPR/Cas9 technique was successfully used to edit the
genome of the obligatory outcrossing plant species Medicago sativa L.(alfalfa). RNA-guided …

Genomic manipulation is essential to the use of model organisms to understand
development, regeneration and adult physiology. The axolotl (Ambystoma mexicanum), a …

The CRISPR/Cas9 technique is widely used in experimentation with human cell lines as
well as with other model systems, such as mice Mus musculus, zebrafish Danio reiro, and …

The neural crest (NC) is a vertebrate-specific cell type that contributes to a wide range of
different tissues across all three germ layers. The gene regulatory network (GRN) …

Background Zebrafish have practical features that make them a useful model for higher-
throughput tests of gene function using CRISPR/Cas9 editing to create 'knockout'models. In …

RAD51 is an indispensable homologous recombination protein, necessary for strand
invasion and crossing over. It has recently been designated as a Fanconi anemia (FA) gene …

Abstract Pyridox (am) ine 5′-phosphate oxidase (PNPO) catalyzes oxidation of pyridoxine
5′-phosphate (PNP) and pyridoxamine 5′-phosphate (PMP) to pyridoxal 5′-phosphate …