Collagen fibrils in the extracellular matrix allow connective tissues such as tendon, skin and
bone to withstand tensile forces. The fibrils are indeterminate in length, insoluble and form …

Newly synthesized secretory cargo molecules pass through the Golgi apparatus while
resident Golgi proteins remain in the organelle. However, the pathways of membrane traffic …

ATR controls chromosome integrity and chromatin dynamics. We have previously shown
that yeast Mec1/ATR promotes chromatin detachment from the nuclear envelope to …

Super-resolution microscopy requires that subcellular structures are labeled with bright and
photostable fluorophores, especially for live-cell imaging. Organic fluorophores may help …

A variety of secretory cargoes move through the Golgi, but the pathways and mechanisms of
this traffic are still being debated. Here, we evaluate the strengths and weaknesses of five …

We have developed a simple correlative photooxidation method that allows for the direct
ultrastructural visualization of the green fluorescent protein (GFP) upon illumination. The …

The Golgi complex is the central sorting and processing station of the secretory pathway,
ensuring that cargo proteins, which are synthesized in the endoplasmic reticulum, are …

COPI-coated vesicles are protein and liquid carriers that mediate transport within the early
secretory pathway. In this Topical Review, we present their main protein components and …

Proteins essential for vesicle formation by the Coat Protein I (COPI) complex are being
identified, but less is known about the role of specific lipids. Brefeldin-A ADP-ribosylated …

The Golgi apparatus contains multiple classes of cisternae that differ in structure,
composition, and function, but there is no consensus about the number and definition of …