▪ Abstract Plant transformation is now a core research tool in plant biology and a practical
tool for cultivar improvement. There are verified methods for stable introduction of novel …

DNA transfer by particle bombardment makes use of physical processes to achieve the
transformation of crop plants. There is no dependence on bacteria, so the limitations …

A collection of Arabidopsis lines with T-DNA insertions in known sites was generated to
increase the efficiency of functional genomics. A high-throughput modified thermal asymetric …

Two rapid and simple in planta transformation methods have been developed for the model
legume Medicago truncatula. The first approach is based on a method developed for …

To study the influence of genomic context on transgene expression, we have determined the
T-DNA structure, flanking DNA sequences, and chromosomal location of four independent …

T-DNA flanking sequences were isolated from 112 Arabidopsis thaliana single-copy T-DNA
lines and sequence mapped to the chromosomes. Even though two T-DNA insertions …

Agrobacterium tumefaciens can transfer part of its Ti plasmid, the T-DNA, to plant cells
where it integrates into the nuclear genome via illegitimate recombination. Integration of the …

Plants are particularly attractive as large-scale production systems for proteins intended for
therapeutical or industrial applications: they can be grown easily and inexpensively in large …

T-DNA is commonly used for delivery of foreign genes and as an insertional mutagen.
Although ample information exists regarding T-DNA organization in dicotyledonous plants …

In a screen for leaf developmental mutants we have isolated an activator T-DNA-tagged
mutant that produces leaves without a petiole. In addition to that leafy petiole phenotype this …