The emerging inverse electron demand Diels–Alder (IEDDA) reaction stands out from other
bioorthogonal reactions by virtue of its unmatchable kinetics, excellent orthogonality and …

Recently, click chemistry has provided important advances in biomedical research fields.
Particularly, copper-free click chemistry including strain-promoted azide–alkyne …

Bioorthogonal chemistry has enabled the selective labeling and detection of biomolecules in
living systems. Bioorthogonal smart probes, which become fluorescent or deliver imaging or …

Fluorogenic probes for bioorthogonal labeling chemistry are highly beneficial to reduce
background signal in fluorescence microscopy imaging. 1, 2, 4, 5-Tetrazines are known …

Small-molecule fluorophores enable the observation of biomolecules in their native context
with fluorescence microscopy. Specific labeling via bio-orthogonal tetrazine chemistry …

Recent developments in fluorescence microscopy call for novel small‐molecule‐based
labels with multiple functionalities to satisfy different experimental requirements. A current …

Vertebrate glycans constitute a large, important, and dynamic set of post‐translational
modifications that are notoriously difficult to manipulate and image. Although the chemical …

Bioorthogonal reactions have been widely used over the last 10 years for imaging,
detection, diagnostics, drug delivery, and biomaterials. Tetrazine reactions are a recently …

Understanding how organelles interact, exchange materials, assemble, disassemble, and
evolve as a function of space, time, and environment is an exciting area at the very forefront …

A series of double-fluorogenic siliconrhodamine probes were synthesized. These tetrazine-
functionalized, membrane-permeable labels allowed site-specific bioorthogonal tagging of …