The main objectives of bioprocesses are to reliably deliver drugs in a relatively short time
frame with high quality within a tight regulatory framework. Bioprocesses are highly complex …

A novel approach of design of experiment (DoE) is developed for the optimization of key
substrates of the culture medium, amino acids, and sugars, by utilizing perfusion …

In this paper, we demonstrate the coupling of synchrotron small angle X-ray scattering
(SAXS) to asymmetrical flow-field flow fractionation (AF4) for protein characterization. To the …

Replacing batch unit operations of biopharmaceuticals by continuous manufacturing is a
maturing concept, with periodic counter-current chromatography (PCC) favoured to replace …

BACKGROUND Tangential flow filtration (TFF) systems are widely used cell retention
devices in perfusion cultures, but significant challenges occur with their prolonged …

Oligo-deoxythymidine (oligo-dT) ligand-based affinity chromatography is a robust method for
purifying mRNA drug substances within the manufacturing process of mRNA-based …

Buffer management for biopharmaceutical purification processes include buffer preparation,
storage of buffers and restocking the buffers when needed. This is usually performed …

The biopharmaceutical market is driving the revolution from traditional batch processes to
continuous manufacturing for higher productivity and lower costs. In this work, a batch mAb …

The implementation of continuous processing in the biopharmaceutical industry is hindered
by the scarcity of process analytical technologies (PAT). To monitor and control a continuous …

Abstract Development of integrated, continuous biomanufacturing (ICB) processes brings
along the challenge of streamlining the acquisition of data that can be used for process …