Capillary electrophoresis is recognized as a valued separation technique for its high
separation efficiency, low sample consumption, good economic and ecological aspects …

This review is in support of the development of selective, precise, fast, and validated
capillary electrophoresis (CE) methods. It follows up a similar article from 1998, Wätzig H …

The current state of the art of CE instruments and their performance have been reviewed,
considering precision, speed, resolution, linearity, sensitivity, ease of use, flexibility …

Proteins are efficient therapeutic agents and attractive potential new biomarkers due to their
physiological and pathological functions. Further, biopharmaceuticals feature complex …

Therapeutic antibodies are paramount in treating a wide range of diseases, particularly in
auto-immunity, inflammation and cancer, and novel antibody candidates recognizing a vast …

The characterization of cysteine-linked antibody‒drug conjugates (ADCs) can be more
challenging than that of monoclonal antibodies (mAbs) and lysine-linked ADCs because the …

Native mass spectrometry (nMS) is a cutting‐edge technique that leverages electrospray
ionization MS (ESI‐MS) to investigate large biomolecules and their complexes in solution …

Charge heterogeneity is inherent to all therapeutic antibodies and arises from post-
translational modifications (PTMs) and/or protein degradation events that may occur during …

An imaged capillary isoelectric focusing (icIEF)‐based method was developed and validated
as a multi‐attribute method for a bispecific antibody (BsAb). First, as the traditional …

CONCLUSIONS This work demonstrates a microfluidic chip-based icIEF-UV/MS technology
deployed in 14 different settings (different locations, systems, and users) and used to …