kallisto, bustools and kb-python for quantifying bulk, single-cell and single-nucleus RNA-seq

DK Sullivan, KH Min, KE Hjörleifsson, L Luebbert… - Nature …, 2024 - nature.com
The term 'RNA-seq'refers to a collection of assays based on sequencing experiments that
involve quantifying RNA species from bulk tissue, single cells or single nuclei. The kallisto …

Best practices for the execution, analysis, and data storage of plant single-cell/nucleus transcriptomics

C Grones, T Eekhout, D Shi, M Neumann… - The Plant …, 2024 - academic.oup.com
Single-cell and single-nucleus RNA-sequencing technologies capture the expression of
plant genes at an unprecedented resolution. Therefore, these technologies are gaining …

simpleaf: a simple, flexible, and scalable framework for single-cell data processing using alevin-fry

D He, R Patro - Bioinformatics, 2023 - academic.oup.com
The alevin-fry ecosystem provides a robust and growing suite of programs for single-cell
data processing. However, as new single-cell technologies are introduced, as the …

Reciprocal crosstalk between Th17 and mesothelial cells promotes metastasis‐associated adhesion of ovarian cancer cells

F Neuhaus, S Lieber, V Shinkevich… - Clinical and …, 2024 - Wiley Online Library
Abstract Background IL‐17A and TNF synergistically promote inflammation and
tumorigenesis. Their interplay and impact on ovarian carcinoma (OC) progression are …

[HTML][HTML] Universal preprocessing of single-cell genomics data

AS Booeshaghi, DK Sullivan, L Pachter - bioRxiv, 2023 - ncbi.nlm.nih.gov
We describe a workflow for preprocessing a wide variety of single-cell genomics data types.
The approach is based on parsing of machine-readable seqspec assay specifications to …

Flexible parsing and preprocessing of technical sequences with splitcode

DK Sullivan, L Pachter - bioRxiv, 2023 - biorxiv.org
Next-generation sequencing libraries are constructed with numerous synthetic constructs
such as sequencing adapters, barcodes, and unique molecular identifiers. Such sequences …

Assessing the multimodal tradeoff

AS Booeshaghi, F Gao, L Pachter - BioRxiv, 2021 - biorxiv.org
Single-cell and single-nucleus genomics assays are becoming increasingly complex, with
multiple measurements of distinct modalities performed concurrently resulting in …

[HTML][HTML] Flexible parsing, interpretation, and editing of technical sequences with splitcode

DK Sullivan, L Pachter - Bioinformatics, 2024 - academic.oup.com
Results We present a tool called splitcode, that enables flexible and efficient parsing,
interpreting, and editing of sequencing reads. This versatile tool facilitates simple …

Algorithms for a Commons Cell Atlas

AS Booeshaghi, Á Gálvez-Merchán, L Pachter - bioRxiv, 2024 - biorxiv.org
Cell atlas projects curate representative datasets, cell types, and marker genes for tissues
across an organism. Despite their ubiquity, atlas projects rely on duplicated and manual …