Insulin secretion is a highly dynamic process regulated by various factors including
nutrients, hormones, and neuronal inputs. The dynamics of insulin secretion can be studied …

The release of insulin from pancreatic islets requires negative regulation to ensure low
levels of insulin release under resting conditions, as well as positive regulation to facilitate …

Background The ability to deliver a gene of interest into a specific cell type is an essential
aspect of biomedical research. Viruses can be a useful tool for this delivery, particularly in …

Pancreatic islet β cells secrete insulin in response to nutrient secretagogues, like glucose,
dependent on calcium influx and nutrient metabolism. One of the most intriguing qualities of …

Controlled matrix interactions were presented to pancreatic β-cells in three-dimensional
culture within poly (ethylene glycol) hydrogels. Dispersed MIN6 β-cells were encapsulated …

The influence of matrix-derived adhesive peptide sequences on encapsulated β-cell survival
and glucose-stimulated insulin release was explored by covalently incorporating synthetic …

Technological developments make mass spectrometry (MS)-based proteomics a central
pillar of biochemical research. MS has been very successful in cell culture systems, where …

The maintenance of whole-body glucose homeostasis is critical for survival, and is
controlled by the coordination of multiple organs and endocrine systems. Pancreatic islet β …

Exocytosis of insulin is dependent on the soluble N-ethylmaleimide attachment protein
receptor (SNARE) complex proteins in the B-cells. We assessed insulin release as well as …

Insulin-stimulated GLUT4 translocation is central to glucose homeostasis. Functional assays
to distinguish individual steps in the GLUT4 translocation process are lacking, thus limiting …