Droplet-based microfluidics

T Moragues, D Arguijo, T Beneyton, C Modavi… - Nature Reviews …, 2023 - nature.com
Droplet-based microfluidic systems generate, manipulate and control sub-microlitre droplets
enclosed within an immiscible carrier fluid. Owing to a number of remarkable features, such …

Recent trends in biocatalysis

D Yi, T Bayer, CPS Badenhorst, S Wu… - Chemical Society …, 2021 - pubs.rsc.org
Biocatalysis has undergone revolutionary progress in the past century. Benefited by the
integration of multidisciplinary technologies, natural enzymatic reactions are constantly …

Ultrahigh-Throughput Enzyme Engineering and Discovery in In Vitro Compartments

M Gantz, S Neun, EJ Medcalf, LD van Vliet… - Chemical …, 2023 - ACS Publications
Novel and improved biocatalysts are increasingly sourced from libraries via experimental
screening. The success of such campaigns is crucially dependent on the number of …

Advances in ultrahigh-throughput screening for directed enzyme evolution

U Markel, KD Essani, V Besirlioglu, J Schiffels… - Chemical Society …, 2020 - pubs.rsc.org
Enzymes are versatile catalysts and their synthetic potential has been recognized for a long
time. In order to exploit their full potential, enzymes often need to be re-engineered or …

Recent advances in droplet microfluidics

Y Ding, PD Howes, AJ deMello - Analytical chemistry, 2019 - ACS Publications
Prominent within this technology set are droplet-based microfluidic systems, 3 in which
passive microfluidic structures are used to rapidly generate and manipulate subnanoliter …

Droplet-based microfluidics in biomedical applications

L Amirifar, M Besanjideh, R Nasiri, A Shamloo… - …, 2022 - iopscience.iop.org
Droplet-based microfluidic systems have been employed to manipulate discrete fluid
volumes with immiscible phases. Creating the fluid droplets at microscale has led to a …

Machine learning for microfluidic design and control

D McIntyre, A Lashkaripour, P Fordyce, D Densmore - Lab on a Chip, 2022 - pubs.rsc.org
Microfluidics has developed into a mature field with applications across science and
engineering, having particular commercial success in molecular diagnostics, next …

Screening and characterization of a diverse panel of metagenomic imine reductases for biocatalytic reductive amination

JR Marshall, P Yao, SL Montgomery, JD Finnigan… - Nature …, 2021 - nature.com
Finding faster and simpler ways to screen protein sequence space to enable the
identification of new biocatalysts for asymmetric synthesis remains both a challenge and a …

Flow cytometry: the next revolution

JP Robinson, R Ostafe, SN Iyengar, B Rajwa, R Fischer - Cells, 2023 - mdpi.com
Unmasking the subtleties of the immune system requires both a comprehensive knowledge
base and the ability to interrogate that system with intimate sensitivity. That task, to a …

High-throughput screening by droplet microfluidics: Perspective into key challenges and future prospects

EM Payne, DA Holland-Moritz, S Sun, RT Kennedy - Lab on a Chip, 2020 - pubs.rsc.org
In two decades of development, impressive strides have been made for automating basic
laboratory operations in droplet-based microfluidics, allowing the emergence of a new form …