It is commonly known that enzymatic transformations are considerably more specific than
classical chemical reactions which usually lead to formation of byproducts. That is why the …

The ability to rapidly and efficiently digest and identify an unknown protein is of great utility
for proteome studies. Identification of proteins via peptide mapping is generally …

There is an urgent need for quantitative assays in verifying and validating the large numbers
of protein biomarker candidates produced in modern"-omics" experiments. Stable isotope …

Microelectromechanical systems (MEMS) are evolving into highly integrated technologies
for a variety of application areas. Add the biological dimension to the mix and a host of new …

Proteolytic digestion of proteins in seconds under an ultrasonic field provided by high-
intensity focused ultrasound (HIFU) has been achieved. Successful in-solution and in-gel …

Mass spectrometry-based identification of the components of multiprotein complexes often
involves solution-phase proteolytic digestion of the complex. The affinity purification of …

In shotgun proteomics, a complex protein mixture is digested to peptides, separated, and
identified by microcapillary liquid chromatography coupled with tandem mass spectrometry …

Capillary enzymatic microreactors containing trypsin and endoproteinase LysC immobilized
on a porous polymer monolith have been prepared and used for the characterization and …

Microfluidic devices with a dual function containing both a solid-phase extractor and an
enzymatic microreactor have been prepared, and their operation has been demonstrated …

In this work, a novel and facile route was developed for the immobilization of enzyme on
nanosized magnetic particles, and its application to fast protein digestion via a direct MALDI …