Tyrosine-type site-specific recombinases (T-SSRs) have opened new avenues for the
predictable modification of genomes as they enable precise genome editing in heterologous …

Gene therapy by engineered nucleases is a genetic intervention being investigated for
curing the hereditary disorders by targeting selected genes with specific nucleotides for …

Targeted nucleases, including zinc-finger nucleases (ZFNs), transcription activator-like
(TAL) effector nucleases (TALENs) and clustered regularly interspaced short palindromic …

Tools for rapid and efficient transgenesis in" safe harbor" loci in an isogenic context remain
important to exploit the possibilities of human pluripotent stem cells (hPSCs). We created …

Widely observed dysregulation of microRNAs (miRNAs) in human cancer has led to
substantial speculation regarding possible functions of these short, non-coding RNAs in …

Articular cartilage covers the ends of bone and provides shock absorption and lubrication to
the diarthrodial joints. Cartilage has a limited capacity for repair when injured, and there is a …

Derivation of hypoimmunogenic human cells from genetically manipulated pluripotent stem
cells holds great promise for future transplantation medicine and adoptive immunotherapy …

In the decade since Yamanaka and colleagues described methods to reprogram somatic
cells into a pluripotent state, human induced pluripotent stem cells (hiPSCs) have …

Background Redirection of natural killer (NK) cells with chimeric antigen receptors (CAR) is
attractive in developing off-the-shelf CAR therapeutics for cancer treatment. However, the …

CRISPR technology is rapidly evolving, and the scope of CRISPR applications is constantly
expanding. CRISPR was originally employed for genome editing. Its application was then …