Cryo-electron microscopy (cryo-EM) is rapidly becoming an attractive method in the field of
structural biology. With the exploding popularity of cryo-EM, sample preparation must evolve …

Membrane proteins are highly diverse in both structure and function and can, therefore,
present different challenges for structure determination. They are biologically important for …

Cryo-electron microscopy (cryo-EM) has the capacity to capture molecular machines in
action,–. ATP-binding cassette (ABC) exporters are highly dynamic membrane proteins that …

Integral membrane proteins (IMPs) fulfill important physiological functions by providing cell–
environment, cell–cell and virus–host communication; nutrients intake; export of toxic …

Highlights•Cryo-EM is a powerful tool to investigate the structures of membrane
proteins.•Recent technological advancements in the field of cryo-EM have enabled structure …

Integral membrane proteins (IMPs) are biologically highly significant but challenging to study
because they require maintaining a cellular lipid-like environment. Here, we explore the …

In recent years, cryo electron microscopy (cryo-EM) technology has been transformed with
the development of better instrumentation, direct electron detectors, improved methods for …

Simple Summary Membrane proteins are essential to all forms of life. Millions of membrane
proteins are found in the lipid membrane layer that surrounds cells, and in the lipid …

Membrane proteins are essential for cellular growth, signalling and homeostasis, making up
a large proportion of therapeutic targets. However, the necessity for a solubilising agent to …

In the recent years, the protein databank has been fueled by the exponential growth of high-
resolution electron cryo-microscopy (cryo-EM) structures. This trend will be further …