Two decades of describing the unseen majority of aquatic microbial diversity

L Zinger, A Gobet, T Pommier - Molecular ecology, 2012 - Wiley Online Library
Aquatic environments harbour large and diverse microbial populations that ensure their
functioning and sustainability. In the current context of global change, characterizing …

A systematic review of sources of variability and uncertainty in eDNA data for environmental monitoring

C Mathieu, SM Hermans, G Lear, TR Buckley… - Frontiers in Ecology …, 2020 - frontiersin.org
Environmental DNA (eDNA) is becoming a standard tool in environmental monitoring that
aims to quantify spatiotemporal variation for the measurement and prediction of ecosystem …

ITS as an environmental DNA barcode for fungi: an in silico approach reveals potential PCR biases

E Bellemain, T Carlsen, C Brochmann, E Coissac… - BMC microbiology, 2010 - Springer
Background During the last 15 years the internal transcribed spacer (ITS) of nuclear DNA
has been used as a target for analyzing fungal diversity in environmental samples, and has …

Metatranscriptomic census of active protists in soils

S Geisen, AT Tveit, IM Clark, A Richter… - The ISME …, 2015 - academic.oup.com
The high numbers and diversity of protists in soil systems have long been presumed, but
their true diversity and community composition have remained largely concealed. Traditional …

Diversity in a hidden world: potential and limitation of next‐generation sequencing for surveys of molecular diversity of eukaryotic microorganisms

R Medinger, V Nolte, RV Pandey, S Jost… - Molecular …, 2010 - Wiley Online Library
With the delivery of millions of sequence reads in a single experiment, next‐generation
sequencing (NGS) is currently revolutionizing surveys of microorganism diversity. In …

Protistan microbial observatory in the Cariaco Basin, Caribbean. I. Pyrosequencing vs Sanger insights into species richness

V Edgcomb, W Orsi, J Bunge, S Jeon… - The ISME …, 2011 - academic.oup.com
Microbial diversity and distribution are topics of intensive research. In two companion papers
in this issue, we describe the results of the Cariaco Microbial Observatory (Caribbean Sea …

Overview of freshwater microbial eukaryotes diversity: a first analysis of publicly available metabarcoding data

D Debroas, I Domaizon, JF Humbert… - FEMS Microbiology …, 2017 - academic.oup.com
Although they are widespread, diverse and involved in biogeochemical cycles, microbial
eukaryotes attract less attention than their prokaryotic counterparts in environmental …

Metatranscriptomics reveals the diversity of genes expressed by eukaryotes in forest soils

C Damon, F Lehembre, C Oger-Desfeux, P Luis… - PloS one, 2012 - journals.plos.org
Eukaryotic organisms play essential roles in the biology and fertility of soils. For example the
micro and mesofauna contribute to the fragmentation and homogenization of plant organic …

Not all are free‐living: high‐throughput DNA metabarcoding reveals a diverse community of protists parasitizing soil metazoa

S Geisen, I Laros, A Vizcaíno, M Bonkowski… - Molecular …, 2015 - Wiley Online Library
Protists, the most diverse eukaryotes, are largely considered to be free‐living bacterivores,
but vast numbers of taxa are known to parasitize plants or animals. High‐throughput …

Are we overestimating protistan diversity in nature?

DA Caron, SK Hu - Trends in Microbiology, 2019 - cell.com
Documenting the immense diversity of single-celled, eukaryotic organisms (protists) has
been a formidable challenge for ecologists. These species were originally defined by …