Comprehensive evaluation of methods for small extracellular vesicles separation from human plasma, urine and cell culture medium

L Dong, RC Zieren, K Horie, CJ Kim… - Journal of …, 2020 - Wiley Online Library
One of the challenges that restricts the evolving extracellular vesicle (EV) research field is
the lack of a consensus method for EV separation. This may also explain the diversity of the …

Diagnostic liquid biopsy biomarkers in renal cell cancer

RC Zieren, PJ Zondervan, KJ Pienta, A Bex… - Nature Reviews …, 2024 - nature.com
The clinical presentation of renal cell cancer (RCC) is shifting towards incidental and early
detection, creating new challenges in RCC diagnosis. Overtreatment might be reduced with …

Prognostic value and multifaceted roles of tetraspanin CD9 in cancer

R Ondruššek, B Kvokačková, K Kryštofová… - Frontiers in …, 2023 - frontiersin.org
CD9 is a crucial regulator of cell adhesion in the immune system and plays important
physiological roles in hematopoiesis, blood coagulation or viral and bacterial infections. It is …

Lectins as potential tools for cancer biomarker discovery from extracellular vesicles

MK Islam, M Khan, K Gidwani, KW Witwer… - Biomarker …, 2023 - Springer
Extracellular vesicles (EVs) have considerable potential as diagnostic, prognostic, and
therapeutic agents, in large part because molecular patterns on the EV surface betray the …

Affinity captured urinary extracellular vesicles provide mRNA and miRNA biomarkers for improved accuracy of prostate cancer detection: a pilot study

M Davey, S Benzina, M Savoie, G Breault… - International journal of …, 2020 - mdpi.com
Serum prostate-specific antigen (sPSA) testing has helped to increase early detection of and
decrease mortality from prostate cancer. However, since sPSA lacks specificity, an invasive …

The role of extracellular vesicles in the treatment of prostate cancer

C Hu, Q Chen, T Wu, X Du, Y Dong, Z Peng, W Xue… - Small, 2024 - Wiley Online Library
Prostate cancer (PCa) has become a public health concern in elderly men due to an ever‐
increasing number of estimated cases. Unfortunately, the available treatments are …

Extracellular vesicle isolation from human renal cancer tissue

RC Zieren, L Dong, PM Pierorazio, KJ Pienta… - Medical Oncology, 2020 - Springer
Renal cell carcinoma is a lethal disease that is often discovered incidentally. New non-
invasive biomarkers are needed to aid diagnosis and treatment. Extracellular vesicles (EVs) …

The role of liquid biopsies in prostate cancer management

CJ Kim, L Dong, SR Amend, YK Cho, KJ Pienta - Lab on a Chip, 2021 - pubs.rsc.org
Liquid biopsy has emerged as a complement to invasive tissue biopsy to guide cancer
diagnosis and treatment. The common liquid biopsy biomarkers are circulating tumor cells …

High-throughput simultaneous mRNA profiling using nCounter technology demonstrates that extracellular vesicles contain different mRNA transcripts than their …

L Dong, CY Huang, EJ Johnson, L Yang… - Analytical …, 2021 - ACS Publications
Extracellular vesicles (EVs) are nano-sized lipid bilayer encapsulated particles with a
molecular cargo that appears to play important roles within the human body, such as in cell …

[HTML][HTML] Advancements in the identification of EV derived mRNA biomarkers for liquid biopsy of clear cell renal cell carcinomas

MD Kuczler, RC Zieren, L Dong, TM de Reijke… - Urology, 2022 - Elsevier
Objective To propose EV-derived mRNA as a potential diagnostic biomarker detecting the
presence of clear cell renal cell carcinoma (ccRCC). There is currently no kidney cancer …