This paper considers how enzymes that catalyze reactions at specific DNA sites have been
engineered to overcome the problem of competitive inhibition by excess nonspecific binding …

Investigations of protein-nucleic acid interactions are necessary to fully understand the
molecular details of many cellular processes. Both equilibrium and kinetic considerations …

Thermodynamic studies have demonstrated the central importance of a large negative heat
capacity change (Δ C° assoc) in site-specific protein-DNA recognition. Dissection of the …

This second edition explains the fundamentals of enzymology and describes the role of
enzymes in food, agricultural and health sciences. Among other topics, it provides new …

The site-specific binding interaction of lac repressor with a symmetric operator sequence
and of EcoRI endonuclease with its specific recognition site both exhibit a characteristic …

High sequence selectivity in DNA-protein interactions was analyzed by measuring
discrimination by Eco RI endonuclease between the recognition site GAATTC and …

The crystal structure of the complex between Eco RI endonuclease and the cognate
oligonucleotide TCGCGAATTCGCG provides a detailed example of the structural basis of …

We previously proposed that salicylic acid (SA)-sensitive catalases serve as biological
targets of SA in plant defense responses. To further examine the role of SA-sensitive …

The nuclease activity of 1, 10-phenanthroline-copper ion can be targeted to specific DNA
sequences by attachment of the ligand to the 5'end of complementary …

In this paper, finite-difference solutions to the nonlinear Poisson-Boltzmann (NLPB) equation
are used to calculate the salt dependent contribution to the electrostatic DNA binding free …