A pragmatic guide to enrichment strategies for mass spectrometry–based glycoproteomics

NM Riley, CR Bertozzi, SJ Pitteri - Molecular & Cellular Proteomics, 2021 - ASBMB
Glycosylation is a prevalent, yet heterogeneous modification with a broad range of
implications in molecular biology. This heterogeneity precludes enrichment strategies that …

[HTML][HTML] Recent advances in hydrophilic interaction liquid interaction chromatography materials for glycopeptide enrichment and glycan separation

G Qing, J Yan, X He, X Li, X Liang - TrAC Trends in Analytical Chemistry, 2020 - Elsevier
Glycosylation is one of the most common and important types of protein post-translational
modifications in mammalian cells. To decode the protein glycosylation, it is highly important …

Optimal Dissociation Methods Differ for N- and O-Glycopeptides

NM Riley, SA Malaker, MD Driessen… - Journal of proteome …, 2020 - ACS Publications
Site-specific characterization of glycosylation requires intact glycopeptide analysis, and
recent efforts have focused on how to best interrogate glycopeptides using tandem mass …

MS‐based glycomics and glycoproteomics methods enabling isomeric characterization

W Peng, CD Gutierrez Reyes, S Gautam… - Mass spectrometry …, 2023 - Wiley Online Library
Glycosylation is one of the most significant and abundant posttranslational modifications in
mammalian cells. It mediates a wide range of biofunctions, including cell adhesion, cell …

Recent advances in software tools for more generic and precise intact glycopeptide analysis

W Cao, M Liu, S Kong, M Wu, Y Zhang… - Molecular & Cellular …, 2021 - ASBMB
Intact glycopeptide identification has long been known as a key and challenging barrier to
the comprehensive and accurate understanding the role of glycosylation in an organism …

The benefits of hybrid fragmentation methods for glycoproteomics

KR Reiding, A Bondt, V Franc, AJR Heck - TrAC Trends in Analytical …, 2018 - Elsevier
Glycosylation is an important and variable protein modification that can have a profound
effect on the physiological characteristics of the substrate, warranting careful examination in …

In-depth site-specific analysis of N-glycoproteome in human cerebrospinal fluid and glycosylation landscape changes in Alzheimer's disease

Z Chen, Q Yu, Q Yu, J Johnson, R Shipman… - Molecular & Cellular …, 2021 - ASBMB
As the body fluid that directly interchanges with the extracellular fluid of the central nervous
system (CNS), cerebrospinal fluid (CSF) serves as a rich source for CNS-related disease …

Comprehensive structural glycomic characterization of the glycocalyxes of cells and tissues

Q Li, Y Xie, M Wong, M Barboza, CB Lebrilla - Nature Protocols, 2020 - nature.com
The glycocalyx comprises glycosylated proteins and lipids and fcorms the outermost layer of
cells. It is involved in fundamental inter-and intracellular processes, including non-self-cell …

Recent advances in analytical approaches for glycan and glycopeptide quantitation

DG Delafield, L Li - Molecular & Cellular Proteomics, 2021 - ASBMB
Growing implications of glycosylation in physiological occurrences and human disease have
prompted intensive focus on revealing glycomic perturbations through absolute and relative …

GPSeeker enables quantitative structural N-glycoproteomics for site-and structure-specific characterization of differentially expressed N-glycosylation in hepatocellular …

K Xiao, Z Tian - Journal of proteome research, 2019 - ACS Publications
N-Glycosylation, being one of the most common and complex protein post-translational
modifications (PTMs), is known to have microheterogeneity with the presence of different N …