Neurogenesis is defined as a process that includes the proliferation of neural
stem/progenitor cells (NPCs) and the differentiation of these cells into new neurons that …

BACKGROUND: Mutations in transcription factor NKX2. 5 cause congenital heart disease
(CHD). We identified a CHD family with atrial septal defects (ASDs), atrioventricular block …

Cancer is one of the most serious leading causes of death in the world. Many eclectic factors
are involved in cancer progression including genetic and epigenetic alongside …

Inhibition of TRPML1, which is encoded by MCOLN1, is known to deter cell proliferation in
various malignancies. Here, we report that the tumor suppressor, p53, represses MCOLN1 …

In this article, we describe a high-throughput cloning method, seamless enzyme-free cloning
(SEFC), which allows one-step assembly of DNA fragments in vivo via homologous …

MicroRNAs (miRNAs) are small endogenous RNAs molecules, approximately 21–23
nucleotides in length, which regulate gene expression by base-pairing with 3′ untranslated …

Background Plasmid construction of small fragments of interest (such as insertion of small
fragment marker genes, expression of shRNA, siRNA, etc) is the basis of many biomolecular …

Multiple gene silencing is being required to target and tangle metabolic pathways in
eukaryotes and researchers have to develop a subtle method for construction of RNA …

Background: Short hairpin RNA (shRNA) has proven to be a powerful tool to study genes'
function through RNA interference mechanism. Three different methods have been used in …

Background CPEB is considered as an RNA-binding protein first identified in Xenopus
oocytes. Although CPEB1 was involved in the growth of oocyte, its role in goat follicular …