Several proteomics approaches are available that are defined by the level (protein or
peptide) at which analysis takes place. The most widely applied method still is bottom-up …

Fast, efficient characterization of proteins is becoming one of the hottest topics in the
bioanalytical community, especially for large-scale proteomic studies. As an attractive …

There is an urgent need for quantitative assays in verifying and validating the large numbers
of protein biomarker candidates produced in modern"-omics" experiments. Stable isotope …

Proteolytic digestion of proteins in seconds under an ultrasonic field provided by high-
intensity focused ultrasound (HIFU) has been achieved. Successful in-solution and in-gel …

A novel kind of immobilized trypsin reactor based on organic− inorganic hybrid silica
monoliths has been developed. With the presence of cetyltrimethyl ammonium bromide …

Capillary enzymatic microreactors containing trypsin and endoproteinase LysC immobilized
on a porous polymer monolith have been prepared and used for the characterization and …

The combinations of gel electrophoresis or LC and mass spectrometry are two popular
approaches for large scale protein identification. However, the throughput of both …

In this work, a novel and facile route was developed for the immobilization of enzyme on
nanosized magnetic particles, and its application to fast protein digestion via a direct MALDI …

Recent tools addressed to accelerate the different steps of the sample treatment for protein
identification in modern workflows are reviewed and critically commented in this manuscript …

With the acceleration of proteome research, increasing attention has been paid to
multidimensional liquid chromatography–mass spectrometry (MDLC–MS) due to its high …