Engineering pyrrolysine systems for genetic code expansion and reprogramming
DL Dunkelmann, JW Chin - Chemical Reviews, 2024 - ACS Publications
Over the past 16 years, genetic code expansion and reprogramming in living organisms has
been transformed by advances that leverage the unique properties of pyrrolysyl-tRNA …
been transformed by advances that leverage the unique properties of pyrrolysyl-tRNA …
Building biomaterials through genetic code expansion
Genetic code expansion (GCE) enables directed incorporation of noncoded amino acids
(NCAAs) and unnatural amino acids (UNAAs) into the active core that confers dedicated …
(NCAAs) and unnatural amino acids (UNAAs) into the active core that confers dedicated …
Secondary amine catalysis in enzyme design: broadening protein template diversity through genetic code expansion
TL Williams, IM Taily, L Hatton, AA Berezin… - Angewandte …, 2024 - Wiley Online Library
Secondary amines, due to their reactivity, can transform protein templates into catalytically
active entities, accelerating the development of artificial enzymes. However, existing …
active entities, accelerating the development of artificial enzymes. However, existing …
An evolved pyrrolysyl-tRNA synthetase with polysubstrate specificity expands the toolbox for engineering enzymes with incorporation of noncanonical amino acids
Aminoacyl-tRNA synthetase (aaRS) is a core component for genetic code expansion (GCE),
a powerful technique that enables the incorporation of noncanonical amino acids (ncAAs) …
a powerful technique that enables the incorporation of noncanonical amino acids (ncAAs) …
Different Techniques of Genetic Engineering Used for the Development of Novel Biomaterials
A Chhina, V Sachdeva, S Thakur - Engineered Biomaterials: Synthesis …, 2023 - Springer
Biomaterials are garnering huge success in the modern era of unforeseen disease
conditions. They find their utility in specifically interacting with biological systems for …
conditions. They find their utility in specifically interacting with biological systems for …
Break the template boundary: Test of different protein scaffolds by genetic code expansion resulted in NADPH-dependent secondary amine catalysis
T Williams, YH Tsai, L Luk - 2021 - researchsquare.com
Here, incorporation of secondary amine by genetic code expansion was used to expand the
potential protein templates for artificial enzyme design. Pyrrolysine analogue containing a D …
potential protein templates for artificial enzyme design. Pyrrolysine analogue containing a D …