Challenges and opportunities in cryo-EM single-particle analysis

D Lyumkis - Journal of Biological Chemistry, 2019 - ASBMB
Cryogenic electron microscopy (cryo-EM) enables structure determination of
macromolecular objects and their assemblies. Although the techniques have been …

Radiation damage to organic and inorganic specimens in the TEM

RF Egerton - Micron, 2019 - Elsevier
Symptoms of radiation damage are reviewed, followed by a brief description of the three
main damage mechanisms: knock-on displacement (predominant in electrically conducting …

EMPIAR: the electron microscopy public image archive

A Iudin, PK Korir, S Somasundharam… - Nucleic Acids …, 2023 - academic.oup.com
Public archiving in structural biology is well established with the Protein Data Bank (PDB;
wwPDB. org) catering for atomic models and the Electron Microscopy Data Bank (EMDB; …

New tools for automated high-resolution cryo-EM structure determination in RELION-3

J Zivanov, T Nakane, BO Forsberg, D Kimanius… - elife, 2018 - elifesciences.org
Here, we describe the third major release of RELION. CPU-based vector acceleration has
been added in addition to GPU support, which provides flexibility in use of resources and …

Real-time cryo-electron microscopy data preprocessing with Warp

D Tegunov, P Cramer - Nature methods, 2019 - nature.com
The acquisition of cryo-electron microscopy (cryo-EM) data from biological specimens must
be tightly coupled to data preprocessing to ensure the best data quality and microscope …

cisTEM, user-friendly software for single-particle image processing

T Grant, A Rohou, N Grigorieff - elife, 2018 - elifesciences.org
We have developed new open-source software called cis TEM (computational imaging
system for transmission electron microscopy) for the processing of data for high-resolution …

MotionCor2: anisotropic correction of beam-induced motion for improved cryo-electron microscopy

SQ Zheng, E Palovcak, JP Armache, KA Verba… - Nature …, 2017 - nature.com
MotionCor2: anisotropic correction of beam-induced motion for improved cryo-electron
microscopy | Nature Methods Skip to main content Thank you for visiting nature.com. You are …

Bisalt ether electrolytes: a pathway towards lithium metal batteries with Ni-rich cathodes

J Alvarado, MA Schroeder, TP Pollard… - Energy & …, 2019 - pubs.rsc.org
The electrochemical performance and mechanistic effects of incorporating two salts in an
ether electrolyte in Li–metal cells were investigated experimentally and via molecular scale …

[HTML][HTML] Gctf: Real-time CTF determination and correction

K Zhang - Journal of structural biology, 2016 - Elsevier
Accurate estimation of the contrast transfer function (CTF) is critical for a near-atomic
resolution cryo electron microscopy (cryoEM) reconstruction. Here, a GPU-accelerated …

Cryo-EM in drug discovery: achievements, limitations and prospects

JP Renaud, A Chari, C Ciferri, W Liu… - Nature reviews Drug …, 2018 - nature.com
Cryo-electron microscopy (cryo-EM) of non-crystalline single particles is a biophysical
technique that can be used to determine the structure of biological macromolecules and …