Protein engineering for improving and diversifying natural product biosynthesis
Proteins found in nature have traditionally been the most frequently used biocatalysts to
produce numerous natural products ranging from commodity chemicals to pharmaceuticals …
produce numerous natural products ranging from commodity chemicals to pharmaceuticals …
Advanced strategies for production of natural products in yeast
R Chen, S Yang, L Zhang, YJ Zhou - Iscience, 2020 - cell.com
Natural products account for more than 50% of all small-molecule pharmaceutical agents
currently in clinical use. However, low availability often becomes problematic when a …
currently in clinical use. However, low availability often becomes problematic when a …
Oxetane ring formation in taxol biosynthesis is catalyzed by a bifunctional cytochrome P450 enzyme
Taxol is a potent drug used in various cancer treatments. Its complex structure has prompted
extensive research into its biosynthesis. However, certain critical steps, such as the …
extensive research into its biosynthesis. However, certain critical steps, such as the …
Engineering yeast for high-level production of diterpenoid sclareol
The diterpenoid sclareol is an industrially important precursor for alternative sustainable
supply of ambergris. However, its current production from plant extraction is neither …
supply of ambergris. However, its current production from plant extraction is neither …
Expanding the terpene biosynthetic code with non-canonical 16 carbon atom building blocks
Humankind relies on specialized metabolites for medicines, flavors, fragrances, and
numerous other valuable biomaterials. However, the chemical space occupied by …
numerous other valuable biomaterials. However, the chemical space occupied by …
Tailoring the Saccharomyces cerevisiae endoplasmic reticulum for functional assembly of terpene synthesis pathway
The endoplasmic reticulum (ER) is a dynamic organelle that synthesizes and folds proteins.
An imbalance between the ER protein synthesis load and its folding capacity triggers the …
An imbalance between the ER protein synthesis load and its folding capacity triggers the …
Engineering yeast for the production of plant terpenoids using synthetic biology approaches
Covering: 2011–2022 The low amounts of terpenoids produced in plants and the difficulty in
synthesizing these complex structures have stimulated the production of terpenoid …
synthesizing these complex structures have stimulated the production of terpenoid …
Recent advances in synthetic biology for engineering isoprenoid production in yeast
Highlights•Isoprenoids are industrially important natural products.•Yeast are key industrial
production vehicles.•New synthetic biology approaches in recent years are reviewed.•Future …
production vehicles.•New synthetic biology approaches in recent years are reviewed.•Future …
Identifying and engineering the ideal microbial terpenoid production host
S Moser, H Pichler - Applied Microbiology and Biotechnology, 2019 - Springer
More than 70,000 different terpenoid structures are known so far; many of them offer highly
interesting applications as pharmaceuticals, flavors and fragrances, or biofuels. Extraction of …
interesting applications as pharmaceuticals, flavors and fragrances, or biofuels. Extraction of …
Engineering chimeric diterpene synthases and isoprenoid biosynthetic pathways enables high-level production of miltiradiene in yeast
Miltiradiene is a key intermediate in the biosynthesis of many important natural diterpene
compounds with significant pharmacological activity, including triptolide, tanshinones …
compounds with significant pharmacological activity, including triptolide, tanshinones …