Exploring the applications of plant-based coagulants in cheese production: A review

AZ Mohsin, E Norsah, AA Marzlan… - International Dairy …, 2024 - Elsevier
Common milk coagulants, such as calf rennet, genetically engineered chymosin, and
microbial rennet, are costly and face restrictions due to ethical, cultural, and dietary …

Plant-derived rennet: research progress, novel strategies for their isolation, identification, mechanism, bioactive peptide generation, and application in cheese …

X Zhang, L Tao, G Wei, M Yang, Z Wang… - Critical Reviews in …, 2025 - Taylor & Francis
Rennet, an aspartate protease found in the stomach of unweaned calves, effectively cuts the
peptide bond between Phe105-Met106 in κ-casein, hydrolyzing the casein micelles to …

Use of glyoxyl-agarose immobilized ficin extract in milk coagulation: Unexpected importance of the ficin loading on the biocatalysts

EH Siar, R Morellon-Sterling, MN Zidoune… - International journal of …, 2020 - Elsevier
A protein extract obtained from fig tree (Ficus carica) latex containing ficin activity was
immobilized on glyoxyl agarose. Different biocatalyst loadings were used (3, 10, 30 and 85 …

[PDF][PDF] Pharmacological profile of Calotropis gigantea in various diseases: A profound look

H Kumar, S Sharma, N Vasudeva - Int J Creat Res Thoughts, 2021 - researchgate.net
Plants are used by peoples for the basis for medical treatments from ancient time and are
still in routinely used as traditional medicine in practiced today. There are a huge number of …

Optimization of three-phase partitioning system for the purification of protease from Calotropis gigantea latex using response surface methodology

B Maskey, DB Karki, NK Shrestha - Chemical Papers, 2024 - Springer
The response surface methodology (RSM) was utilized to optimize three-phase partitioning
(TPP) parameters for the purification of Calotropis gigantea latex protease. The limits of …

Exploring cheese production enzymes from various plants as an alternative to Calf rennet

AT Tesfaw, YM Sewmehon, AT Tegegne, GB Alemu… - Discover Food, 2024 - Springer
Milk coagulation, traditionally relied on Calf rennet, is a crucial enzymatic process in cheese
production. Ethical and practical constraints in using Calf rennet have led to the exploration …

Potential utility of callus proteases as a milk clotting alternative to naturally propagated Wrightia tinctoria proteases

A Rajagopalan, V Aluru, M Soundarajan… - Preparative …, 2022 - Taylor & Francis
This study was intended to understand suitability of Wrightia tinctoria calli proteases (CCE)
as vegetable coagulant through delineating their milk clotting potential and casein (whole …

Effect of purified Calotropis gigantea latex protease on the cheese quality

NK Shrestha, B Maskey - Tribhuvan University Journal of Food Science …, 2023 - nepjol.info
Cheesemaking has been relying solely on chymosin for so long. But factors like increased
cheese production, diminishing supply of chymosin as well as religious and dietary …

[PDF][PDF] Optimization of Protease Enzyme Extraction from Calatropis Procera Using Aqueous Two-Phase Purification

AB Braimah, D Ackah, P Manu - Science and Development Journal, 2023 - ajol.info
Though protease enzyme extraction has mainly been from microbes, plant proteases have
been sought out as a supplement to further boost production and overcome the expensive …

Clotting properties of Onopordum tauricum aqueous extract in milk of different species

K DAVYDZENKA - 2019 - tesi.univpm.it
Abstract in italiano Plant coagulants have become a subject of growing interest in cheese
industry due to their easy availability and simple purification process. The milk clotting …