Point-of-care CRISPR/Cas nucleic acid detection: Recent advances, challenges and opportunities

JE van Dongen, JTW Berendsen… - Biosensors and …, 2020 - Elsevier
With the trend of moving molecular tests from clinical laboratories to on-site testing, there is a
need for nucleic acid based diagnostic tools combining the sensitivity, specificity and …

Exploring the trans‐cleavage activity of CRISPR‐Cas12a (cpf1) for the development of a universal electrochemical biosensor

Y Dai, RA Somoza, L Wang, JF Welter, Y Li… - Angewandte …, 2019 - Wiley Online Library
An accurate, rapid, and cost‐effective biosensor for the quantification of disease biomarkers
is vital for the development of early‐diagnostic point‐of‐care systems. The recent discovery …

eccDNAs are apoptotic products with high innate immunostimulatory activity

Y Wang, M Wang, MN Djekidel, H Chen, D Liu, FW Alt… - Nature, 2021 - nature.com
Extrachromosomal circular DNA elements (eccDNAs) have been described in the literature
for several decades, and are known for their broad existence across different species …

An amplification-free ultra-sensitive electrochemical CRISPR/Cas biosensor for drug-resistant bacteria detection

A Suea-Ngam, PD Howes, AJ DeMello - Chemical science, 2021 - pubs.rsc.org
Continued development of high-performance and cost-effective in vitro diagnostic tools is
vital for improving infectious disease treatment and transmission control. For nucleic acid …

Signal Amplification by the trans-Cleavage Activity of CRISPR-Cas Systems: Kinetics and Performance

W Feng, H Zhang, XC Le - Analytical Chemistry, 2023 - ACS Publications
Nucleic acid cleavage processes contribute to target and signal amplification, which is
critical for the development of highly sensitive assays. 1, 2 Exonucleases or endonucleases …

Alternative functions of CRISPR–Cas systems in the evolutionary arms race

P Mohanraju, C Saha, P van Baarlen… - Nature Reviews …, 2022 - nature.com
CRISPR–Cas systems of bacteria and archaea comprise chromosomal loci with typical
repetitive clusters and associated genes encoding a range of Cas proteins. Adaptation of …

Surpassing the detection limit and accuracy of the electrochemical DNA sensor through the application of CRISPR Cas systems

W Xu, T Jin, Y Dai, CC Liu - Biosensors and Bioelectronics, 2020 - Elsevier
Robust developments of personalized medicine for next-generation healthcare highlight the
need for sensitive and accurate point-of-care platforms for quantification of disease …

MeCas12a, a highly sensitive and specific system for COVID‐19 detection

P Ma, Q Meng, B Sun, B Zhao, L Dang… - Advanced …, 2020 - Wiley Online Library
Cas12a‐based systems, which detect specific nucleic acids via collateral cleavage of
reporter DNA, display huge potentials for rapid diagnosis of infectious diseases. Here, the …

CRISPR-based COVID-19 testing: toward next-generation point-of-care diagnostics

U Ganbaatar, C Liu - Frontiers in cellular and infection microbiology, 2021 - frontiersin.org
As the COVID-19 pandemic continues, people are becoming infected at an alarming rate,
individuals are unknowingly spreading disease, and more lives are lost every day. There is …

CRISPR-Cas12a has widespread off-target and dsDNA-nicking effects

K Murugan, AS Seetharam, AJ Severin… - Journal of Biological …, 2020 - ASBMB
Cas12a (Cpf1) is an RNA-guided endonuclease in the bacterial type VA CRISPR-Cas anti-
phage immune system that can be repurposed for genome editing. Cas12a can bind and cut …