The communication between cells and between cellular organelles is often controlled by the
interaction of membrane proteins. Although many methods for the detection of protein …

Regulated proteolysis is essential for the normal physiology of all organisms. While all
eukaryotes and archaea use proteasomes for protein degradation, only certain orders of …

Prokaryotes form ubiquitin (Ub)-like isopeptide bonds on the lysine residues of proteins by at
least two distinct pathways that are reversible and regulated. In mycobacteria, the C-terminal …

Interest in bacterial proteasomes was sparked by the discovery that proteasomal
degradation is required for the pathogenesis of Mycobacterium tuberculosis, one of the …

Protein‐protein interactions (PPIs) play critical roles in almost all cellular signal transduction
events. Characterization of PPIs without interfering with the functions of intact cells is very …

Tuberculosis is caused by the bacterium Mycobacterium tuberculosis (Mtb) and is ranked as
the second killer infectious disease after COVID-19. Proteasome accessory factor A (PafA) is …

Post-synthesis DNA modification is a very useful method for DNA functionalization. This is
achieved by using a modified NTP, which has a handle for further modifications, replacing …

The proper functioning of any biological system depends on the coordinated activity of its
components. Regulation at the genetic level is, in many cases, effective in determining the …

Abstract Systems analogous to the eukaryotic ubiquitin-proteasome system have been
previously identified in Archaea, and Actinobacteria (gram-positive), but not in gram …

Bacteria belonging to the phyla Actinobacteria and Nitrospira possess proteasome cores
homologous to the eukaryotic 20S proteasome particle. In these bacteria, the cytoplasmic …