Understanding the invisible hands of sample preparation for cryo-EM
Cryo-electron microscopy (cryo-EM) is rapidly becoming an attractive method in the field of
structural biology. With the exploding popularity of cryo-EM, sample preparation must evolve …
structural biology. With the exploding popularity of cryo-EM, sample preparation must evolve …
Subatomic species transport through atomically thin membranes: Present and future applications
BACKGROUND Membranes are thin physical barriers that allow for transport of certain
species while limiting others. Atomically thin two-dimensional (2D) materials are crystalline …
species while limiting others. Atomically thin two-dimensional (2D) materials are crystalline …
Atomically precise nanoclusters predominantly seed gold nanoparticle syntheses
Seed-mediated synthesis strategies, in which small gold nanoparticle precursors are added
to a growth solution to initiate heterogeneous nucleation, are among the most prevalent …
to a growth solution to initiate heterogeneous nucleation, are among the most prevalent …
Direct visualization of ligands on gold nanoparticles in a liquid environment
The interactions between gold nanoparticles, their surface ligands and the solvent critically
influence the properties of these nanoparticles. Although spectroscopic and scattering …
influence the properties of these nanoparticles. Although spectroscopic and scattering …
Cryo-EM structure determination of small proteins by nanobody-binding scaffolds (Legobodies)
X Wu, TA Rapoport - … of the National Academy of Sciences, 2021 - National Acad Sciences
We describe a general method that allows structure determination of small proteins by single-
particle cryo-electron microscopy (cryo-EM). The method is based on the availability of a …
particle cryo-electron microscopy (cryo-EM). The method is based on the availability of a …
Membranes under the magnetic lens: a dive into the diverse world of membrane protein structures using Cryo-EM
Membrane proteins are highly diverse in both structure and function and can, therefore,
present different challenges for structure determination. They are biologically important for …
present different challenges for structure determination. They are biologically important for …
Cryo-EM analysis of a membrane protein embedded in the liposome
Membrane proteins (MPs) used to be the most difficult targets for structural biology when X-
ray crystallography was the mainstream approach. With the resolution revolution of single …
ray crystallography was the mainstream approach. With the resolution revolution of single …
Structural conservation among variants of the SARS-CoV-2 spike postfusion bundle
Variants of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) challenge
currently available COVID-19 vaccines and monoclonal antibody therapies due to structural …
currently available COVID-19 vaccines and monoclonal antibody therapies due to structural …
Molecular architecture of the Chikungunya virus replication complex
To better understand how positive-strand (+) RNA viruses assemble membrane-associated
replication complexes (RCs) to synthesize, process, and transport viral RNA in virus-infected …
replication complexes (RCs) to synthesize, process, and transport viral RNA in virus-infected …
Synthesis of wafer‐scale graphene with chemical vapor deposition for electronic device applications
The first isolation of graphene opens the avenue for new platforms for physics, electronic
engineering, and materials sciences. Among several kinds of synthesis approaches …
engineering, and materials sciences. Among several kinds of synthesis approaches …