Bioorthogonal chemistry is a set of methods using the chemistry of non-native functional
groups to explore and understand biology in living organisms. In this review, we summarize …

Chemically modified biomacromolecules─ ie, proteins, nucleic acids, glycans, and lipids─
have become crucial tools in chemical biology. They are extensively used not only to …

The merging of click chemistry with discrete photochemical processes has led to the creation
of a new class of click reactions, collectively known as photoclick chemistry. These light …

Triazines are an important class of six-membered aromatic heterocycles possessing three
nitrogen atoms, resulting in three types of regio-isomers: 1, 2, 4-triazines (a-triazines), 1, 2, 3 …

Reactions that occur under physiological conditions find diverse uses in the chemical and
biological sciences. However, the limitations that biological systems place on chemical …

The two main strategies for enzyme engineering, directed evolution and rational design,
have found widespread applications in improving the intrinsic activities of proteins. Although …

Electrophilic small molecules that can reversibly modify proteins are of growing interest in
drug discovery. However, the ability to study reversible covalent probes in live cells can be …

Advancement of bioorthogonal chemistry in molecular optical imaging lies in expanding the
repertoire of fluorophores that can undergo fluorescence signal changes upon …

Click chemistry has become one of the most powerful construction tools in the field of
organic chemistry, materials science, and polymer science, as it offers hassle-free platforms …

Described is the spatiotemporally controlled labeling and patterning of biomolecules in live
cells through the catalytic activation of bioorthogonal chemistry with light, referred to as …