Single-molecule biophysics has transformed our understanding of biology, but also of the
physics of life. More exotic than simple soft matter, biomatter lives far from thermal …

Conspectus Methods for detecting and quantifying disease biomarkers in biofluids with high
specificity and sensitivity play a pivotal role in enabling clinical diagnostics, including point …

Abstract Background The Random Forest (RF) algorithm for supervised machine learning is
an ensemble learning method widely used in science and many other fields. Its popularity …

Single-molecule Förster Resonance energy transfer (smFRET) is an adaptable method for
studying the structure and dynamics of biomolecules. The development of high throughput …

Fluorescent in-situ hybridization (FISH)-based methods extract spatially resolved genetic
and epigenetic information from biological samples by detecting fluorescent spots in …

Traces from single-molecule fluorescence microscopy (SMFM) experiments exhibit
photophysical artifacts that typically necessitate human expert screening, which is time …

Optical spectroscopy and imaging techniques play important roles in many fields such as
disease diagnosis, biological study, information technology, optical science, and materials …

Interactions occurring at the nuclear envelope (NE)–chromatin interface influence both NE
structure and chromatin organization. Insights into the functions of NE–chromatin …

Abstract The MED-1, 2 GATA factors contribute to specification of E, the progenitor of the
Caenorhabditis elegans endoderm, through the genes end-1 and end-3, and in parallel with …

Cis-regulatory elements (CREs) are crucial links in developmental gene regulatory
networks, but in many cases, it can be difficult to discern whether similar CREs are …