Cardiovascular diseases represent a major cause of morbidity and mortality, necessitating
research to improve diagnostics, and to discover and test novel preventive and curative …

Base editing is a powerful CRISPR-based technology for introducing precise substitutions
into the genome. This technology greatly advances mutagenesis possibilities in vivo …

Zebrafish are an established research organism that has made many contributions to our
understanding of vertebrate tissue and organ development, yet there are still significant …

The reliance on viral vectors for the production of genetically engineered immune cells for
adoptive cellular therapies remains a translational bottleneck. Here we report a method …

Efficient and precise targeted insertion holds great promise but remains challenging in plant
genome editing. An efficient nonhomologous end-joining-mediated targeted insertion …

Base Editors are emerging as an innovative technology to introduce point mutations in
complex genomes. So far, the requirement of an NGG Protospacer Adjacent Motif (PAM) at a …

Despite the unprecedented gene editing capability of CRISPR-Cas9-mediated targeted
knock-in, the efficiency and precision of this technology still require further optimization …

The African turquoise killifish is a powerful vertebrate system to study complex phenotypes
at scale, including aging and age-related disease. Here, we develop a rapid and precise …

The CRISPR/Cas9 system has been used to generate fluorescently labelled fusion proteins
by homology-directed repair in a variety of species. Despite its revolutionary success, there …

Cancer cells exist in a complex ecosystem with numerous other cell types in the tumor
microenvironment (TME). The composition of this tumor/TME ecosystem will vary at each …