Rising CO2 emissions have pushed scientists to develop new technologies for a more
sustainable bio-based economy. Microbial conversion of CO2 and CO2-derived carbon …

Highlights•Synthetic methanotrophy systems for chemical production are discussed.•Current
CH 4-derived chemical production in methanotrophs is not efficient.•Engineering CH4 …

Synthetic biology aims at designing new biological functions from first principles. These new
designs allow to expand the natural solution space and overcome the limitations of naturally …

The remarkable metabolic diversity observed in nature has provided a foundation for
sustainable production of a wide array of valuable molecules. However, transferring the …

One-carbon (C1) compounds are emerging as cost-effective and potentially carbon-negative
feedstocks for biomanufacturing, which require efficient, versatile metabolic platforms for the …

Formic acid (FA) has emerged as a promising one-carbon feedstock for biorefinery.
However, developing efficient microbial hosts for economically competitive FA utilization …

Achieving cost-competitive bio-based processes requires development of stable and
selective biocatalysts. Their realization through in vitro enzyme characterization and …

Synthetic biology is being increasingly used to establish novel carbon assimilation pathways
and artificial autotrophic strains that can be used in low-carbon biomanufacturing. Currently …

Highlights•C1 compounds can be converted into formaldehyde.•Formaldehyde can be
condensed into multicarbon products by C1 carboligases.•Enzyme and metabolic …

Formaldehyde is an intermediate metabolite of methylotrophic microorganisms that can be
obtained from formate and methanol through oxidation–reduction reactions. Formaldehyde …