Tissue culture-independent approaches to revolutionizing plant transformation and gene editing

LF Quiroz, M Khan, N Gondalia, L Lai… - Horticulture …, 2024 - academic.oup.com
Despite the transformative power of gene editing for crop improvement, its widespread
application across species and varieties is limited by the transformation bottleneck that …

A comprehensive review of in planta stable transformation strategies

JG Bélanger, TR Copley, V Hoyos-Villegas, JB Charron… - Plant Methods, 2024 - Springer
Plant transformation remains a major bottleneck to the improvement of plant science, both
on fundamental and practical levels. The recalcitrant nature of most commercial and minor …

Functional analysis of RsWUSb with Agrobacterium-mediated in planta transformation in radish (Raphanus sativus L.)

Q Hu, J Dong, J Ying, Y Wang, L Xu, Y Ma, L Wang… - Scientia …, 2024 - Elsevier
The WUSCHEL-related homeobox (WOX) family genes are involved in cell division and
differentiation throughout various stages of plant growth and development. Till now, the …

Overexpression of AtMYB12 transcription factor simultaneously enhances quercetin-dependent metabolites in radish callus

M Balasubramanian, S Girija - Heliyon, 2024 - cell.com
The study aimed to enhance quercetin production in radish by optimizing Agrobacterium
tumefaciens-mediated in-planta transformation. This protocol involved infecting radish seed …

Comparative Evaluation of Transient Protein Expression Efficiency in Tissues across Soybean Varieties Using the Tsukuba System

MB Fuhrmann-Aoyagi, S Igarashi, K Miura - Plants, 2024 - mdpi.com
Transient protein expression is a versatile tool with diverse applications and can be used in
soybeans to study gene function, obtain mutants, and produce proteins for commercial use …

A novel approach for direct shoot regeneration, anatomical characterization, and withanolides content in micropropagated plants of Withania somnifera (L.) Dunal—an …

G Mahendran - In Vitro Cellular & Developmental Biology-Plant, 2024 - Springer
Withania somnifera (Ashwagandha) is a valuable therapeutic plant used in many Ayurvedic
drugs marketed for health restoration. However, its commercial production faces challenges …

Meta-topolin enhances regeneration and Agrobacterium-mediated genetic transformation in radish (Raphanus sativus L.)

S Karthik, S Sathish, JJ Sahayarayan… - In Vitro Cellular & …, 2022 - Springer
Meta-topolin (m T) is a novel aromatic cytokinin that stimulates morphogenesis and is an
alternative source of cytokinins frequently employed in regeneration systems. Hence, the …

Improved efficiency of callus induction, bud differentiation, and plant regeneration in radish (Raphanus sativus L.)

CM Li, FB Chen, CY Wang, Y Liu… - New Zealand Journal of …, 2025 - Taylor & Francis
Radish (Raphanus sativus L.) is a recalcitrant variety for embryogenic callus induction that
does not result in an efficient genetic transformation system. In this study, a highly efficient …

Radish (Raphanus sativus L.) as an object of Agrobacterium-mediated transformation

XA Kuznetsova, IE Dodueva… - Ecological …, 2024 - journals.eco-vector.com
Radish (Raphanus sativus L.) is an annual root crop closely related to Arabidopsis thaliana.
It can be widely used as a perspective model plant to study the genetic control of storage …