The emergence of CRISPR-Cas systems has accelerated the development of gene editing
technologies, which are widely used in the life sciences. To improve the performance of …

Cellular pathways that repair chromosomal double-strand breaks (DSBs) have pivotal roles
in cell growth, development and cancer. These DSB repair pathways have been the target of …

The study of model organisms has revolutionized our understanding of the mechanisms
underlying normal development, adult homeostasis, and human disease. Much of what we …

The CRISPR system is widely used in genome editing for biomedical research. Here, using
either dual paired Cas9D10A nickases or paired Cas9 nuclease we characterize …

The CRISPR-Cas system has evolved into a cutting-edge technology that has transformed
the field of biological sciences through precise genetic manipulation. CRISPR/Cas9 …

The genome editing technique CRISPR/Cas9 has led to major advancements in many
research fields and this state-of-the-art tool has proven its use in genetic studies for various …

In recent years, Setaria viridis has been developed as a model plant to better understand the
C4 photosynthetic pathway in major crops. With the increasing availability of genomic …

CRISPR/Cas9 genome editing has revolutionized functional genomics in vertebrates.
However, CRISPR/Cas9 edited F0 animals too often demonstrate variable phenotypic …

phenyl 2-thiourea (PTU) is a Tyr (tyrosinase) inhibitor that is extensively used to block
pigmentation and improve optical transparency in zebrafish (Danio rerio) embryo. Here, we …

Gene editing and/or allele introgression with absolute precision and control appear to be the
ultimate goals of genetic engineering. Precision genome editing in plants has been …