Advances in capillary electrophoresis and the implications for drug discovery

CM Ouimet, CI D'amico, RT Kennedy - Expert opinion on drug …, 2017 - Taylor & Francis
Introduction: Many screening platforms are prone to assay interferences that can be avoided
by directly measuring the target or enzymatic product. Capillary electrophoresis (CE) and …

Advances in capillary electrophoresis-based enzyme assays

GKE Scriba, F Belal - Chromatographia, 2015 - Springer
Capillary electrophoresis (CE) has become a flexible and accurate, high-efficiency analytical
separation technique in many areas requiring only minute amounts of sample and …

Recent advances in CE‐mediated microanalysis for enzyme study

X Wang, K Li, E Adams, AV Schepdael - Electrophoresis, 2014 - Wiley Online Library
This review gives an overview of the recent developments and applications in the use of CE‐
mediated microanalysis for enzyme studies. The period covers mid‐2011 until mid‐2013 …

Tyrosinase inhibitor screening in traditional Chinese medicines by electrophoretically mediated microanalysis

L Tang, W Zhang, H Zhao… - Journal of Separation …, 2015 - Wiley Online Library
A capillary‐electrophoresis‐based method for the screening of tyrosinase inhibitors in
traditional Chinese medicines was developed. The method integrated electrophoretically …

An online immobilized α-glucosidase microreactor for enzyme kinetics and inhibition assays

DM Liu, J Chen, YP Shi - RSC advances, 2015 - pubs.rsc.org
In this work, a facile protocol for preparing an online immobilized α-glucosidase microreactor
was described, and also the enzyme kinetics and inhibition kinetics of such an immobilized …

Human protein kinase inhibitor screening by capillary electrophoresis using transverse diffusion of laminar flow profiles for reactant mixing

H Nehmé, R Nehmé, P Lafite, S Routier… - … of Chromatography A, 2013 - Elsevier
A capillary electrophoresis (CE)-based enzyme assay method has been developed to
screen protein kinase inhibitors. Four human kinases GSK3β, DYRK1A, CDK5/p25 and …

Screening of α-glucosidase inhibitors from natural flavonoids by an in-capillary assay combining PMMA and EMMA

HH Zhao, YQ Liu, J Chen - Analytical Methods, 2019 - pubs.rsc.org
A capillary electrophoresis method combining pressure mediated microanalysis (PMMA)
and electrophoretically mediated microanalysis (EMMA) was established and applied to the …

Integrated microreactor for enzymatic reaction automation: An easy step toward the quality control of monoclonal antibodies

Y Ladner, S Mas, G Coussot, K Bartley… - … of Chromatography A, 2017 - Elsevier
The main purpose of the present work is to provide a fully integrated miniaturized
electrophoretic methodology in order to facilitate the quality control of monoclonal antibodies …

Application of capillary electrophoresis in enzyme inhibitors screening

LIU Dong-Mei, SHI Yan-Ping, C Juan - Chinese Journal of Analytical …, 2015 - Elsevier
Capillary electrophoresis plays an irreplaceable role in the field of enzyme inhibitors
screening with unique features of high-efficiency separation, short analysis time, minimal …

Capillary electrophoresis as a novel technique for screening natural flavonoids as kinase inhibitors

R Nehmé, H Nehmé, G Roux, E Destandau… - … of Chromatography A, 2013 - Elsevier
Capillary electrophoresis (CE) was used for the first time to evaluate the inhibition activity of
aglycone flavonoids (such as quercetin and isorhamnetin) and some of their glycosylated …