Eukaryotic Pif1 helicase unwinds G-quadruplex and dsDNA using a conserved wedge
Abstract G-quadruplexes (G4s) formed by guanine-rich nucleic acids induce genome
instability through impeding DNA replication fork progression. G4s are stable DNA …
instability through impeding DNA replication fork progression. G4s are stable DNA …
[HTML][HTML] Nuclear Structure, Size Regulation, and Role in Cell Migration
Y Li, S Ge, J Liu, D Sun, Y Xi, P Chen - Cells, 2024 - mdpi.com
The nucleus serves as a pivotal regulatory and control hub in the cell, governing numerous
aspects of cellular functions, including DNA replication, transcription, and RNA processing …
aspects of cellular functions, including DNA replication, transcription, and RNA processing …
Genome-wide conditional degron libraries for functional genomics
E Gameiro, KA Juárez-Núñez, JJ Fung… - Journal of Cell …, 2025 - rupress.org
Functional genomics with libraries of knockout alleles is limited to non-essential genes and
convoluted by the potential accumulation of suppressor mutations in knockout backgrounds …
convoluted by the potential accumulation of suppressor mutations in knockout backgrounds …
Two residues in the DNA binding site of Pif1 helicase are essential for nuclear functions but dispensable for mitochondrial respiratory growth
J Gao, DR Proffitt, JC Marecki, RU Protacio… - Nucleic Acids …, 2024 - academic.oup.com
Pif1 helicase functions in both the nucleus and mitochondria. Pif1 tightly couples ATP
hydrolysis, single-stranded DNA translocation, and duplex DNA unwinding. We investigated …
hydrolysis, single-stranded DNA translocation, and duplex DNA unwinding. We investigated …
Alternative translation initiation by ribosomal leaky scanning produces multiple isoforms of the Pif1 helicase
T Lama-Diaz, MG Blanco - Nucleic Acids Research, 2024 - academic.oup.com
In budding yeast, the integrity of both the nuclear and mitochondrial genomes relies on dual-
targeted isoforms of the conserved Pif1 helicase, generated by alternative translation …
targeted isoforms of the conserved Pif1 helicase, generated by alternative translation …
[PDF][PDF] The Nucleus and Its Physiological Role
Y Li, S Ge, J Liu, D Sun, P Chen - 2024 - preprints.org
The nucleus, serving as a pivotal regulatory and control hub within the cell, governs
numerous aspects of cellular functions, including DNA replication, transcription, RNA …
numerous aspects of cellular functions, including DNA replication, transcription, RNA …