Electron microscopy analysis of ATP-independent nucleosome unfolding by FACT

AL Sivkina, MG Karlova, ME Valieva… - Communications …, 2022 - nature.com
FACT is a histone chaperone that participates in nucleosome removal and reassembly
during transcription and replication. We used electron microscopy to study FACT, FACT …

Mechanism of FACT removal from transcribed genes by anticancer drugs curaxins

HW Chang, ME Valieva, A Safina, RV Chereji… - Science …, 2018 - science.org
Human FACT (facilitates chromatin transcription) is a multifunctional protein complex that
has histone chaperone activity and facilitates nucleosome survival and transcription through …

Human PARP1 facilitates transcription through a nucleosome and histone displacement by Pol II in vitro

EY Kotova, FK Hsieh, HW Chang… - International Journal of …, 2022 - mdpi.com
Human poly (ADP)-ribose polymerase-1 (PARP1) is a global regulator of various cellular
processes, from DNA repair to gene expression. The underlying mechanism of PARP1 …

Mechanisms of nucleosome reorganization by PARP1

NV Maluchenko, DK Nilov, SV Pushkarev… - International Journal of …, 2021 - mdpi.com
Poly (ADP-ribose) polymerase 1 (PARP1) is an enzyme involved in DNA repair, chromatin
organization and transcription. During transcription initiation, PARP1 interacts with gene …

Reconstitution and purification of nucleosomes with recombinant histones and purified DNA

IM Nodelman, A Patel, RF Levendosky… - Current protocols in …, 2020 - Wiley Online Library
Nucleosomes are substrates for a broad range of factors, including those involved in
transcription or chromosome maintenance/reorganization and enzymes that covalently …

[HTML][HTML] Resveratrol Inhibits Nucleosome Binding and Catalytic Activity of PARP1

DO Koshkina, NV Maluchenko, AN Korovina… - Biomolecules, 2024 - mdpi.com
The natural polyphenol resveratrol is a biologically active compound that interacts with DNA
and affects the activity of some nuclear enzymes. Its effect on the interaction between …

Stabilization of nucleosomes by histone tails and by FACT revealed by spFRET microscopy

ME Valieva, NS Gerasimova, KS Kudryashova… - Cancers, 2017 - mdpi.com
A correct chromatin structure is important for cell viability and is tightly regulated by
numerous factors. Human protein complex FACT (facilitates chromatin transcription) is an …

Preparation of mononucleosomal templates for analysis of transcription with RNA polymerase using spFRET

KS Kudryashova, OV Chertkov, DV Nikitin… - Chromatin …, 2015 - Springer
Single positioned nucleosomes have been extensively employed as simple model
experimental systems for analysis of various intranuclear processes. Here we describe an …

Hmo1 protein affects the nucleosome structure and supports the nucleosome reorganization activity of yeast FACT

DK Malinina, AL Sivkina, AN Korovina, LL McCullough… - Cells, 2022 - mdpi.com
Yeast Hmo1 is a high mobility group B (HMGB) protein that participates in the transcription of
ribosomal protein genes and rDNA, and also stimulates the activities of some ATP …

Mechanism of curaxin-dependent nucleosome unfolding by FACT

OI Volokh, AL Sivkina, AV Moiseenko… - Frontiers in Molecular …, 2022 - frontiersin.org
Human FACT (FACT) is a multifunctional histone chaperone involved in transcription,
replication and DNA repair. Curaxins are anticancer compounds that induce FACT …