Nearly 80% of the approved human therapeutic antibodies are produced by Chinese
Hamster Ovary (CHO) cells. To achieve better cell growth and high-yield recombinant …

Chinese hamster ovary (CHO) cells can produce proteins with complex structures and post-
translational modifications which are similar to human-derived cells, and they have been the …

Proposed herein is a systematic media design framework that combines multivariate
statistical approaches with in silico analysis of a genome-scale metabolic model of Chinese …

Non-enzymatic hinge fragmentation of monoclonal antibodies (mAb) is considered a critical
quality attribute since it changes the primary sequence of the proteins, thereby leading to …

Rapidly expanding pipelines in biopharmaceutical industry and increasingly stringent
requirements from regulatory agencies call for a more efficient and rational study design at …

Abstract Changes in charge variant profile are known to affect mAb stability and vice versa.
This report elucidates the effects of magnesium metal (0.5 mM Mg 2+) on trastuzumab (IgG1 …

Mammalian cell lines are frequently used as the preferred host cells for producing
recombinant therapeutic proteins (RTPs) having post-translational modified modifications …

Cell culture media play a critical role in cell growth and propagation by providing a
substrate; media components can also modulate the critical quality attributes (CQAs) …

Media preparation parameters contribute significantly to media quality, cell culture
performance, productivity, and product quality. Establishing proper media preparation …

Pressurized intraperitoneal aerosol chemotherapy (PIPAC) is a new therapeutic approach
for patients with peritoneal cancer. So far, most published studies investigated the …