Background Agri-food safety has been considered as one of the most important public
concerns worldwide. From farm to table, food crops and foods are extremely vulnerable to …

Reviewed are nucleic acid detection assays that incorporate clustered regularly interspaced
short palindromic repeats (CRISPR)-based diagnostics and microfluidic devices and …

Strategies utilizing the CRISPR/Cas nucleases Cas13 and Cas12 have shown great
promise in the development of highly sensitive and rapid diagnostic assays for the detection …

CRISPR-Cas12a is an accurate and responsive biosensing technique, but its limited stability
has restricted its widespread applications. To address this, we propose a strategy using …

CRISPR is reshaping biosensing technology due to its programmability, sensitivity, and
specificity. Most current CRISPR-based biosensors are developed based on Cas12 and …

Abstract The fusion of Rolling Circle Amplification (RCA) and the Clustered Regularly
Interspaced Short Palindromic Repeats–CRISPR-associated proteins (CRISPR-Cas) stands …

Foodborne microbial contamination (FMC) is the leading cause of food poisoning and
foodborne illness. The foodborne microbial detection methods based on isothermal …

Abstract Cadmium ion (Cd (II)) is a pernicious environmental pollutant that has been shown
to contaminate agricultural lands, accumulate through the food chain, and seriously threaten …

Flap endonuclease I (FEN1) is a structure-specific nuclease that participates in DNA repair,
and its overexpression is related to an increased risk of cancer. Currently, few FEN1 assays …

Protamine, an arginine-rich polycationic peptide, is a natural food preservative and the only
approved antidote to heparin overdose. The detection of protamine is meaningful; however …