Light sheet fluorescence microscopy (LSFM) uses a thin sheet of light to excite only
fluorophores within the focal volume. Light sheet microscopes (LSMs) have a true optical …

Organisms are inherently three dimensional, thus comprehensive understanding of the
complicated biological system requires analysis of organs or even whole bodies in the …

The contrast and resolution of images obtained with optical microscopes can be improved
by deconvolution and computational fusion of multiple views of the same sample, but these …

Abstract We present Richardson–Lucy network (RLN), a fast and lightweight deep learning
method for three-dimensional fluorescence microscopy deconvolution. RLN combines the …

Fluorescence microscopy has evolved from a purely observational tool to a platform for
quantitative, hypothesis-driven research. As such, the demand for faster and less phototoxic …

The proliferation of microscopy methods for live-cell imaging offers many new possibilities
for users but can also be challenging to navigate. The prevailing challenge in live-cell …

Confocal microscopy remains a major workhorse in biomedical optical microscopy owing to
its reliability and flexibility in imaging various samples, but suffers from substantial point …

Light-sheet fluorescence microscopy (LSFM) has been present in cell biology laboratories
for quite some time, mainly as custom-made systems, with imaging applications ranging …

Diffuse large B-cell lymphoma (DLBCL) can be subdivided into the activated B-cell (ABC)
and germinal center B cell–like (GCB) subtypes. Self-antigen engagement of B-cell …

Fluorescence microscopy is a highly effective tool for interrogating biological structure and
function, particularly when imaging across multiple spatiotemporal scales. Here we survey …