Human brain organogenesis: Toward a cellular understanding of development and disease

KW Kelley, SP Pașca - Cell, 2022 - cell.com
The construction of the human nervous system is a distinctly complex although highly
regulated process. Human tissue inaccessibility has impeded a molecular understanding of …

Engineering brain assembloids to interrogate human neural circuits

Y Miura, MY Li, O Revah, SJ Yoon, G Narazaki… - Nature protocols, 2022 - nature.com
The development of neural circuits involves wiring of neurons locally following their
generation and migration, as well as establishing long-distance connections between brain …

Generation of functional human 3D cortico-motor assembloids

J Andersen, O Revah, Y Miura, N Thom, ND Amin… - Cell, 2020 - cell.com
Neurons in the cerebral cortex connect through descending pathways to hindbrain and
spinal cord to activate muscle and generate movement. Although components of this …

Long-term maturation of human cortical organoids matches key early postnatal transitions

A Gordon, SJ Yoon, SS Tran, CD Makinson… - Nature …, 2021 - nature.com
Human stem-cell-derived models provide the promise of accelerating our understanding of
brain disorders, but not knowing whether they possess the ability to mature beyond mid-to …

Signalling pathways in autism spectrum disorder: mechanisms and therapeutic implications

CC Jiang, LS Lin, S Long, XY Ke, K Fukunaga… - Signal transduction and …, 2022 - nature.com
Autism spectrum disorder (ASD) is a prevalent and complex neurodevelopmental disorder
which has strong genetic basis. Despite the rapidly rising incidence of autism, little is known …

Assembly of functionally integrated human forebrain spheroids

F Birey, J Andersen, CD Makinson, S Islam, W Wei… - Nature, 2017 - nature.com
The development of the nervous system involves a coordinated succession of events
including the migration of GABAergic (γ-aminobutyric-acid-releasing) neurons from ventral …

Reliability of human cortical organoid generation

SJ Yoon, LS Elahi, AM Pașca, RM Marton, A Gordon… - Nature …, 2019 - nature.com
The differentiation of pluripotent stem cells in three-dimensional cultures can recapitulate
key aspects of brain development, but protocols are prone to variable results. Here we …

The rise of three-dimensional human brain cultures

SP Pașca - Nature, 2018 - nature.com
Pluripotent stem cells show a remarkable ability to self-organize and differentiate in vitro in
three-dimensional aggregates, known as organoids or organ spheroids, and to recapitulate …

Calcium channels, synaptic plasticity, and neuropsychiatric disease

E Nanou, WA Catterall - Neuron, 2018 - cell.com
Voltage-gated calcium channels couple depolarization of the cell-surface membrane to entry
of calcium, which triggers secretion, contraction, neurotransmission, gene expression, and …

Human astrocyte maturation captured in 3D cerebral cortical spheroids derived from pluripotent stem cells

SA Sloan, S Darmanis, N Huber, TA Khan, F Birey… - Neuron, 2017 - cell.com
There is significant need to develop physiologically relevant models for investigating human
astrocytes in health and disease. Here, we present an approach for generating astrocyte …