All steps of cryogenic electron-microscopy (cryo-EM) workflows have rapidly evolved over
the last decade. Advances in both single-particle analysis (SPA) cryo-EM and cryo-electron …

The structural determination of biological macromolecules has been transformative for
understanding biochemical mechanisms and developing therapeutics. However, the …

Approximately two-thirds of the estimated one-billion metric tons of methane produced
annually by methanogens is derived from the cleavage of acetate. Acetate is broken down …

Developments within the cryo-EM field have allowed us to generate higher-resolution “static”
structures and pull out different conformational states which exist at equilibrium within the …

Intrinsically disordered regions (IDR) and short linear motifs (SLiMs) play pivotal roles in the
intricate signaling networks governed by phosphatases and kinases. B56δ (encoded by …

The specimen preparation process is a key determinant in the success of any cryo electron
microscopy (cryoEM) structural study and until recently had remained largely unchanged …

Abstract Antibiotic-resistant Neisseria gonorrhoeae (Ng) are an emerging public health
threat due to increasing numbers of multidrug resistant (MDR) organisms. We identified two …

Advances in single particle cryo-EM data collection and processing have seen a significant
rise in its use. However, the influences of the environment generated through grid …

Sample preparation is still a significant problem for many single particle cryo-EM workflows
and our understanding and developments in the area lag behind that of image processing …

Iron-sulfur clusters are essential for life and defects in their biosynthesis lead to human
diseases. The mechanism of cluster assembly and delivery to cytosolic and nuclear client …