Methods of isolating extracellular vesicles impact down-stream analyses of their cargoes

DD Taylor, S Shah - Methods, 2015 - Elsevier
Viable tumor cells actively release vesicles into the peripheral circulation and other biologic
fluids, which exhibit proteins and RNAs characteristic of that cell. Our group demonstrated …

Development of L-asparaginase biobetters: current research status and review of the desirable quality profiles

LP Brumano, FVS da Silva, TA Costa-Silva… - … in bioengineering and …, 2019 - frontiersin.org
L-Asparaginase (ASNase) is a vital component of the first line treatment of acute
lymphoblastic leukemia (ALL), an aggressive type of blood cancer expected to afflict over …

Revealing Nature's Cellulase Diversity: The Digestion Mechanism of Caldicellulosiruptor bescii CelA

R Brunecky, M Alahuhta, Q Xu, BS Donohoe… - Science, 2013 - science.org
Most fungi and bacteria degrade plant cell walls by secreting free, complementary enzymes
that hydrolyze cellulose; however, some bacteria use large enzymatic assemblies called …

Impact of redox-active polymer molecular weight on the electrochemical properties and transport across porous separators in nonaqueous solvents

G Nagarjuna, J Hui, KJ Cheng… - Journal of the …, 2014 - ACS Publications
Enhancing the ionic conductivity across the electrolyte separator in nonaqueous redox flow
batteries (NRFBs) is essential for improving their performance and enabling their …

An efficient method for estimating the hydrodynamic radius of disordered protein conformations

M Nygaard, BB Kragelund, E Papaleo… - Biophysical journal, 2017 - cell.com
Intrinsically disordered proteins play important roles throughout biology, yet our
understanding of the relationship between their sequences, structural properties, and …

Separation, antitumor activities, and encapsulation of polypeptide from Chlorella pyrenoidosa

X Wang, X Zhang - Biotechnology progress, 2013 - Wiley Online Library
Chlorella pyrenoidosa is a unicellular green algae and has been a popular foodstuff
worldwide. However, no reports on the antitumor peptides from such a microalgae are …

Hindered nanoparticle diffusion and void accessibility in a three-dimensional porous medium

MJ Skaug, L Wang, Y Ding, DK Schwartz - ACS nano, 2015 - ACS Publications
The inherent pore-scale heterogeneity of many natural and synthetic porous materials can
make it difficult to model and predict porous transport because the underlying microscopic …

Experimental validation on average conformation of a comblike polystyrene library in dilute solutions: universal scaling laws and abnormal SEC elution behavior

X Pan, M Ding, L Li - Macromolecules, 2021 - ACS Publications
In this work, a sample library of well-defined comblike polystyrenes (PS) has been prepared
for the study of average conformation in dilute solutions. The dependences of radius of …

Shape matters: size-exclusion HPLC for the study of nucleic acid structural polymorphism

E Largy, JL Mergny - Nucleic acids research, 2014 - academic.oup.com
In recent years, an increasing number of reports have been focused on the structure and
biological role of non-canonical nucleic acid secondary structures. Many of these studies …

Extended experimental inferential structure determination method in determining the structural ensembles of disordered protein states

J Lincoff, M Haghighatlari, M Krzeminski… - Communications …, 2020 - nature.com
Proteins with intrinsic or unfolded state disorder comprise a new frontier in structural biology,
requiring the characterization of diverse and dynamic structural ensembles. Here we …